Title:	Exploring anatomical and chemical markers to characterize and differentiate among Bidens species.
Authors:	SABEDOTTI, C. SILVEIRA, I. DA MIGACZ, I. P. CRUZ, L. S. VENTURA, A. C. T. FARAGO, P. V. MONDIN, C. A. RAMAN, V. BRITO, E. S. de RIBEIRO, P. R. V. BELTRAME, F. L. MANFRON, J.
Affiliation:	CAROLINA SABEDOTTI, UNIVERSIDADE ESTADUAL DE PONTA GROSSA; ISABELLA DA SILVEIRA, UNIVERSIDADE ESTADUAL DE PONTA GROSSA; IZABEL PIETCZAK MIGACZ, UNIVERSIDADE ESTADUAL DE PONTA GROSSA; LUIZA STOLZ CRUZ, UNIVERSIDADE ESTADUAL DO CENTRO-OESTE; ANA CAROLINA TERSO VENTURA, UNIVERSIDADE ESTADUAL DE PONTA GROSSA; PAULO VITOR FARAGO, UNIVERSIDADE ESTADUAL DE PONTA GROSSA; CLAUDIO AUGUSTO MONDIN, INSTITUTO CONECTAR AMBIENTAL; VIJAYASANKAR RAMAN, DEPARTMENT OF AGRICULTURE, DISTRICT OF COLUMBIA, NATIONAL IDENTIFICATION SERVICES, WASHINGTON, USA; EDY SOUSA DE BRITO, CNAT; PAULO RICELI VASCONCELOS RIBEIRO, CNPAT; FLÁVIO LUÍS BELTRAME, UNIVERSIDADE ESTADUAL DE PONTA GROSSA; JANE MANFRON, UNIVERSIDADE ESTADUAL DE PONTA GROSSA.
Date Issued:	2026
Citation:	Revista Brasileira de Farmacognosia, v. 36, n. 47, 2026.
Description:	Bidens pilosa L., Bidens alba (L.) DC., and Bidens subalternans DC., Asteraceae, are often misidentified because of their morphological similarities. The present study provides a comparative analysis of the morpho-anatomical characteristics, histochemical, and chemical fingerprints obtained by liquid chromatography mass spectrometry for these three species. Light microscopy, field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy were used to characterize diagnostic structures. Histochemical assays and LC-MS were applied to obtain chemical information from leaf, stem, and root extracts, and multivariate chemometrics including principal component analysis were used to classify the datasets. The morpho-anatomical and histochemical analysis allowed the identification and differentiation of the three species; also, the LC-MS analysis revealed that the main compounds in Bidens extracts are phenolics, and using chemometrics methods was possible to discriminate by species and plant parts. Together, these complementary approaches provide a robust basis for distinguishing closely related Bidens species and support more reliable pharmacognostic identification.
NAL Thesaurus:	Chemotaxonomy Quality control
Keywords:	Herbals Micromorphology Pharmacobotany Species authentication
DOI:	10.1007/s43450-026-00759-8
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNAT)