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dc.contributor.authorAMÂNCIO, B. R.
dc.contributor.authorMAGNANI, E.
dc.contributor.authorNUNES, A. T.
dc.contributor.authorSILVA, T. H.
dc.contributor.authorCORTINHAS, C. S.
dc.contributor.authorCARVALHO, V. V. de
dc.contributor.authorTAMASSIA, L. F. M.
dc.contributor.authorZIHLMANN, R.
dc.contributor.authorBERNDT, A.
dc.contributor.authorSANTOS, J. de O.
dc.contributor.authorCÔNSOLO, N. R. B.
dc.contributor.authorBENEDETI, P. D. B.
dc.contributor.authorARNANDES, R. H. B.
dc.contributor.authorPAULA, E. M.
dc.date.accessioned2026-06-01T20:48:39Z-
dc.date.available2026-06-01T20:48:39Z-
dc.date.created2026-06-01
dc.date.issued2026
dc.identifier.citationJournal of animal science, v. 104, skag068, 2026.
dc.identifier.issn1525-3163
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1187294-
dc.descriptionThis study evaluated the effects of two doses of 3-nitrooxypropanol (3-NOP) on methane (CH4) emissions, performance, dry matter (DM) intake, apparent digestibility, rumen microbiome and metabolomic profile of Nellore cattle fed a high-concentrate finishing finishing diet. Seventy-five 20-month-old Nellore bulls, 361.6 ± 30.08 kg body weight (BW), were individually housed with ad libitum access to feed and water. Animals were distributed in a completely randomized study design, with three treatments and 25 animals per treatment, which were: 1) CON, control (basal diet + mineral premix without 3-NOP), 2) 3-NOP65 (Basal diet + mineral premix + 65 mg 3-NOP/kg of DM), 3) 3-NOP85 (Basal diet + mineral premix + 85 mg 3-NOP/kg of DM). The 115-d trial included a 3-wk adaptation period with increasing dietary concentrate levels from 50% to 88%. Enteric CH4 emissions were measured using the sulfur hexafluoride (SF6) tracer gas technique. Supplementation with 3-NOP had no detrimental effect on final BW (P = 0.89) and average daily gain (ADG; P = 0.94), but DM intake increased linearly with 3-NOP inclusion (P = 0.05). Methane emissions (g/d) were reduced by 13.2% and 26.7% in the 3-NOP65 and 3-NOP85 groups, respectively (P < 0.05), without adverse effects on animal health. Rumen microbiome analysis revealed a quadratic response in the relative abundance of the phylum Euryarchaeota (P = 0.01). Metabolomic analysis indicated significant changes in amino acid and energy metabolism, with proline, arginine, and threonine identified as key discriminant metabolites (VIP > 1) in the 3-NOP85 group. These findings demonstrate that 3-NOP supplementation effectively reduces CH4 emissions in a dose-dependent manner while maintaining animal performance and health.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectSustainability
dc.subjectBovaer
dc.subject3-NOP
dc.titleEffect of two-doses of 3-nitrooxypropanol (3-NOP) on methane emissions, performance, rumen microbiome, and metabolomics in Nellore cattle.
dc.typeArtigo de periódico
dc.subject.thesagroGado de Corte
dc.subject.thesagroBovinocultura
dc.subject.thesagroGado Nelore
dc.subject.thesagroEfeito Estufa
dc.subject.thesagroRúmen
dc.subject.thesagroMetano
dc.subject.nalthesaurusFeed additives
dc.subject.nalthesaurusGreenhouse gases
dc.subject.nalthesaurusMetabolomics
dc.subject.nalthesaurusNellore
dc.subject.nalthesaurusCattle
dc.description.notesNa publicação: Cristina Simões Cortinhas saiu como Cristina Cortinhas, Renata Helena Branco Arnandes saiu como Renata Helena Branco. Financiamento: FAPESP (2017/50339-5, 2018/19743-7, 2019/ 22626-2, 2022/11769-2).
riaa.ainfo.id1187294
riaa.ainfo.lastupdate2026-06-01
dc.identifier.doihttps://doi.org/10.1093/jas/skag068
dc.contributor.institutionBRUNA ROBERTA AMÂNCIO, INSTITUTO DE ZOOTECNIA DO GOVERNO DO ESTADO DE SÃO PAULO; ELAINE MAGNANI, INSTITUTO DE ZOOTECNIA DO GOVERNO DO ESTADO DE SÃO PAULO; ALANNE TENÓRIO NUNES, UNIVERSIDADE DE SÃO PAULO; THIAGO HENRIQUE SILVA, INSTITUTO DE ZOOTECNIA DO GOVERNO DO ESTADO DE SÃO PAULO; CRISTINA SIMÕES CORTINHAS, DSM FIRMENICH; VICTOR VALÉRIO DE CARVALHO, DSM FIRMENICH; LUIS FERNANDO MONTEIRO TAMASSIA, DSM FIRMENICH; RETO ZIHLMANN, DSM FIRMENICH; ALEXANDRE BERNDT, CPPSE; JULIANA DE OLIVEIRA SANTOS MARCATTO, CNPMA; NARA REGINA BRANDÃO CÔNSOLO, UNIVERSIDADE DE SÃO PAULO; PEDRO DEL BIANCO BENEDETI, UNIVERSIDADE DO ESTADO DE SANTA CATARINA; RENATA HELENA BRANCO ARNANDES, INSTITUTO DE ZOOTECNIA DO GOVERNO DO ESTADO DE SÃO PAULO; EDUARDO MAROSTEGAN PAULA, INSTITUTO DE ZOOTECNIA DO GOVERNO DO ESTADO DE SÃO PAULO.
Aparece en las colecciones:Artigo em periódico indexado (CPPSE)


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