Title:	Effect of two-doses of 3-nitrooxypropanol (3-NOP) on methane emissions, performance, rumen microbiome, and metabolomics in Nellore cattle.
Authors:	AMÂNCIO, B. R. MAGNANI, E. NUNES, A. T. SILVA, T. H. CORTINHAS, C. S. CARVALHO, V. V. de TAMASSIA, L. F. M. ZIHLMANN, R. BERNDT, A. SANTOS, J. de O. CÔNSOLO, N. R. B. BENEDETI, P. D. B. ARNANDES, R. H. B. PAULA, E. M.
Affiliation:	BRUNA ROBERTA AMÂNCIO, INSTITUTO DE ZOOTECNIA DO GOVERNO DO ESTADO DE SÃO PAULO; ELAINE MAGNANI, INSTITUTO DE ZOOTECNIA DO GOVERNO DO ESTADO DE SÃO PAULO; ALANNE TENÓRIO NUNES, UNIVERSIDADE DE SÃO PAULO; THIAGO HENRIQUE SILVA, INSTITUTO DE ZOOTECNIA DO GOVERNO DO ESTADO DE SÃO PAULO; CRISTINA SIMÕES CORTINHAS, DSM FIRMENICH; VICTOR VALÉRIO DE CARVALHO, DSM FIRMENICH; LUIS FERNANDO MONTEIRO TAMASSIA, DSM FIRMENICH; RETO ZIHLMANN, DSM FIRMENICH; ALEXANDRE BERNDT, CPPSE; JULIANA DE OLIVEIRA SANTOS MARCATTO, CNPMA; NARA REGINA BRANDÃO CÔNSOLO, UNIVERSIDADE DE SÃO PAULO; PEDRO DEL BIANCO BENEDETI, UNIVERSIDADE DO ESTADO DE SANTA CATARINA; RENATA HELENA BRANCO ARNANDES, INSTITUTO DE ZOOTECNIA DO GOVERNO DO ESTADO DE SÃO PAULO; EDUARDO MAROSTEGAN PAULA, INSTITUTO DE ZOOTECNIA DO GOVERNO DO ESTADO DE SÃO PAULO.
Date Issued:	2026
Citation:	Journal of animal science, v. 104, skag068, 2026.
Description:	This study evaluated the effects of two doses of 3-nitrooxypropanol (3-NOP) on methane (CH4) emissions, performance, dry matter (DM) intake, apparent digestibility, rumen microbiome and metabolomic profile of Nellore cattle fed a high-concentrate finishing finishing diet. Seventy-five 20-month-old Nellore bulls, 361.6 ± 30.08 kg body weight (BW), were individually housed with ad libitum access to feed and water. Animals were distributed in a completely randomized study design, with three treatments and 25 animals per treatment, which were: 1) CON, control (basal diet + mineral premix without 3-NOP), 2) 3-NOP65 (Basal diet + mineral premix + 65 mg 3-NOP/kg of DM), 3) 3-NOP85 (Basal diet + mineral premix + 85 mg 3-NOP/kg of DM). The 115-d trial included a 3-wk adaptation period with increasing dietary concentrate levels from 50% to 88%. Enteric CH4 emissions were measured using the sulfur hexafluoride (SF6) tracer gas technique. Supplementation with 3-NOP had no detrimental effect on final BW (P = 0.89) and average daily gain (ADG; P = 0.94), but DM intake increased linearly with 3-NOP inclusion (P = 0.05). Methane emissions (g/d) were reduced by 13.2% and 26.7% in the 3-NOP65 and 3-NOP85 groups, respectively (P < 0.05), without adverse effects on animal health. Rumen microbiome analysis revealed a quadratic response in the relative abundance of the phylum Euryarchaeota (P = 0.01). Metabolomic analysis indicated significant changes in amino acid and energy metabolism, with proline, arginine, and threonine identified as key discriminant metabolites (VIP > 1) in the 3-NOP85 group. These findings demonstrate that 3-NOP supplementation effectively reduces CH4 emissions in a dose-dependent manner while maintaining animal performance and health.
Thesagro:	Gado de Corte Bovinocultura Gado Nelore Efeito Estufa Rúmen Metano
NAL Thesaurus:	Feed additives Greenhouse gases Metabolomics Nellore Cattle
Keywords:	Sustainability Bovaer 3-NOP
ISSN:	1525-3163
DOI:	https://doi.org/10.1093/jas/skag068
Notes:	Na publicação: Cristina Simões Cortinhas saiu como Cristina Cortinhas, Renata Helena Branco Arnandes saiu como Renata Helena Branco. Financiamento: FAPESP (2017/50339-5, 2018/19743-7, 2019/ 22626-2, 2022/11769-2).
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPPSE)