Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1187457
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorLIMA, N. S. M.
dc.contributor.authorGOMES-PEPE, E. S.
dc.contributor.authorKOCK, F. V. C.
dc.contributor.authorCOLNAGO, L. A.
dc.contributor.authorALVES, P. L. da C. A.
dc.contributor.authorLEMOS, E. G. de M.
dc.date.accessioned2026-06-10T18:48:54Z-
dc.date.available2026-06-10T18:48:54Z-
dc.date.created2026-06-10
dc.date.issued2026
dc.identifier.citationCurrent Microbiology, v. 83, 206, 2026.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1187457-
dc.descriptionThe extensive use of herbicides such as imazapic, from the imidazolinone class, raises environmental concerns due to its persistence and toxicity in ecosystems and subsequent crops. Enzymatic bioremediation emerges as a sustainable alterna tive for the mitigation of these contaminants. This study investigated the potential of the metagenomic laccase, LacMeta, expressed in Escherichia coli BL21 (DE3), to degrade imazapic using a whole-cell approach. LacMeta expression was optimized with CuSO₄, which proved to be four times more effective than IPTG. The E. coli + LacMeta cells demonstrated high tolerance to the herbicide, maintaining cell viability even at high doses (350 g/ha). Notably, the enzymatic activity of LacMeta was not inhibited by imazapic; on the contrary, it was stimulated, reaching a specific activity nearly three times higher in the presence of the herbicide compared to the control. Degradation was confirmed by UV-Visible spec troscopy, which showed the disappearance of imazapic’s characteristic peaks (200–280 nm) over 15 days. ¹H-NMR and FTIR analyses corroborated the degradation, indicating structural changes in the herbicide molecule, particularly in the aromatic ring region (signals at 8.25 and 8.50 ppm). Phytotoxicity assays with lettuce seeds (Lactuca sativa) confirmed that treatment with the LacMeta-containing supernatant cell free significantly reduced the toxicity of imazapic in the soil. The results demonstrate that LacMeta has high potential for the bioremediation of imazapic, and the whole-cell approach represents a promising and cost-effective strategy for the decontamination of environments impacted by this herbicide
dc.language.isoeng
dc.rightsopenAccess
dc.subjectBioremediation agent
dc.subjectImidazolinone class
dc.titleEfficiency of Imazapic Degradation: an Assessment of LacMeta Treatments Utilizing Whole Cell.
dc.typeArtigo de periódico
dc.format.extent210 p.
riaa.ainfo.id1187457
riaa.ainfo.lastupdate2026-06-10
dc.identifier.doihttps://doi.org/10.1007/s00284-026-04760-1
dc.contributor.institutionINSTITUTE FOR RESEARCH IN BIOENERGY (IPBEN), JABOTICABAL; SAO PAULO STATE UNIVERSITY (UNESP); PONTIFICIA UNIVERSIDAD CATÓLICA DEL PERÚ, SAN MIGUEL, LIMA 15088, PERU; LUIZ ALBERTO COLNAGO, CNPDIA; SAO PAULO STATE UNIVERSITY (UNESP); SAO PAULO STATE UNIVERSITY (UNESP).
Aparece nas coleções:Artigo em periódico indexado (CNPDIA)

Arquivos associados a este item:
Arquivo TamanhoFormato 
Efficiency-of-Imazapic-Degradation-an-Assessment-of-LacMeta.pdf2,23 MBAdobe PDFVisualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace