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dc.contributor.authorMATTOS, S. S. F.
dc.contributor.authorSILVA, C. G. da
dc.contributor.authorLIMA, L. B. de
dc.contributor.authorMAIA, L. R.
dc.contributor.authorDILELIS, F.
dc.contributor.authorSILVA, T. E. da
dc.contributor.authorMARTINHO, P. de F.
dc.contributor.authorRUFINO, L. M. de A.
dc.contributor.authorRODRIGUES, J. P. P.
dc.date.accessioned2026-07-28T17:48:47Z-
dc.date.available2026-07-28T17:48:47Z-
dc.date.created2026-07-02
dc.date.issued2026
dc.identifier.citationRevista Brasileira de Zootecnia, v. 55, e20250130, 2026.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1187968-
dc.descriptionThis study aimed to evaluate the potential of using images taken with a smartphone or scanner as alternatives to conventional spectrophotometry for phosphorus (P) analysis of feedstuff. Twenty-four samples of feed ingredients and commercial concentrates were evaluated. After acid digestion and dilution, the solutions were analyzed using the P-molybdate colorimetric reaction. Standards ranged from 0 to 1.4 mg L−1 at intervals of 0.2 mg L−1 of P in the reading solution. Standards and reading solutions were analyzed in ELISA microplates. The absorbance values of each cuvette were obtained using a spectrophotometer, and smartphone and scanner images were obtained sequentially. The images were segmented, and a circle was sampled in each cuvette to obtain the mean values of the red, green, and blue (RGB) channels. The P concentration was determined using linear regression of absorbance or the mean values obtained in the RGB channels as a function of P concentration. The repeatability (REP), limits of detection (LOD), and limits of quantification (LOQ) were calculated. Except for the scanner’s blue channel (P<0.10), image-based colorimetric methods provided similar P concentrations similar to those obtained by spectrophotometry (P>0.10). Using absorbance, the REP, LOD, and LOQ were 0.96%, 0.013, and 0.039 mg L−1, respectively. The best-performing image-based predictors were red and green channels of the smartphone, with REP, LOD, and LOQ values of 1.80%, 0.050, 0.150 mg L−1 and 2.11%, 0.074 and 0.223 mg L−1, respectively. The smartphone red channel spectrophotometric absorbance provided equivalent P concentrations at a 99% confidence interval. Smartphone images performed better than scanner images. The use of the mean red channel from smartphone images proved promising for determining P concentration in feeds.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectAbsorbância
dc.titleColorimetric analysis of phosphorus concentration in animal feeds using images from smartphone or scanner: a proof-of-concept study.
dc.typeArtigo de periódico
dc.subject.thesagroMolibdênio
dc.subject.thesagroAlimentação
dc.subject.thesagroAnálise
dc.subject.nalthesaurusAbsorbance
riaa.ainfo.id1187968
riaa.ainfo.lastupdate2026-07-28
dc.identifier.doihttps://doi.org/10.37496/rbz5520250130
dc.contributor.institutionSUELLEN SOBRINHO FRANÇA MATTOS, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; CRYSTHAL GONÇALVES DA SILVA, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; LORHAINE BERNARDES DE LIMA, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; LARYSSA RODRIGUES MAIA, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; FELIPE DILELIS, UNIVERSIDADE DE SÃO PAULO; TADEU EDER DA SILVA, UNIVERSITY OF VERMONT; PLINIO DE FREITAS MARTINHO, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; LUANA MARTA DE ALMEIDA RUFINO, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; JOAO PAULO PACHECO RODRIGUES, CNPGL.
Appears in Collections:Artigo em periódico indexado (CNPGL)

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