Título:	Colorimetric analysis of phosphorus concentration in animal feeds using images from smartphone or scanner: a proof-of-concept study.
Autor:	MATTOS, S. S. F. SILVA, C. G. da LIMA, L. B. de MAIA, L. R. DILELIS, F. SILVA, T. E. da MARTINHO, P. de F. RUFINO, L. M. de A. RODRIGUES, J. P. P.
Afiliación:	SUELLEN SOBRINHO FRANÇA MATTOS, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; CRYSTHAL GONÇALVES DA SILVA, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; LORHAINE BERNARDES DE LIMA, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; LARYSSA RODRIGUES MAIA, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; FELIPE DILELIS, UNIVERSIDADE DE SÃO PAULO; TADEU EDER DA SILVA, UNIVERSITY OF VERMONT; PLINIO DE FREITAS MARTINHO, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; LUANA MARTA DE ALMEIDA RUFINO, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; JOAO PAULO PACHECO RODRIGUES, CNPGL.
Año:	2026
Referencia:	Revista Brasileira de Zootecnia, v. 55, e20250130, 2026.
Descripción:	This study aimed to evaluate the potential of using images taken with a smartphone or scanner as alternatives to conventional spectrophotometry for phosphorus (P) analysis of feedstuff. Twenty-four samples of feed ingredients and commercial concentrates were evaluated. After acid digestion and dilution, the solutions were analyzed using the P-molybdate colorimetric reaction. Standards ranged from 0 to 1.4 mg L−1 at intervals of 0.2 mg L−1 of P in the reading solution. Standards and reading solutions were analyzed in ELISA microplates. The absorbance values of each cuvette were obtained using a spectrophotometer, and smartphone and scanner images were obtained sequentially. The images were segmented, and a circle was sampled in each cuvette to obtain the mean values of the red, green, and blue (RGB) channels. The P concentration was determined using linear regression of absorbance or the mean values obtained in the RGB channels as a function of P concentration. The repeatability (REP), limits of detection (LOD), and limits of quantification (LOQ) were calculated. Except for the scanner’s blue channel (P<0.10), image-based colorimetric methods provided similar P concentrations similar to those obtained by spectrophotometry (P>0.10). Using absorbance, the REP, LOD, and LOQ were 0.96%, 0.013, and 0.039 mg L−1, respectively. The best-performing image-based predictors were red and green channels of the smartphone, with REP, LOD, and LOQ values of 1.80%, 0.050, 0.150 mg L−1 and 2.11%, 0.074 and 0.223 mg L−1, respectively. The smartphone red channel spectrophotometric absorbance provided equivalent P concentrations at a 99% confidence interval. Smartphone images performed better than scanner images. The use of the mean red channel from smartphone images proved promising for determining P concentration in feeds.
Thesagro:	Molibdênio Alimentação Análise
NAL Thesaurus:	Absorbance
Palabras clave:	Absorbância
DOI:	https://doi.org/10.37496/rbz5520250130
Tipo de Material:	Artigo de periódico
Acceso:	openAccess
Aparece en las colecciones:	Artigo em periódico indexado (CNPGL)