Título:	Compartment-specific responses of soil, root, and earthworm gut microbiomes to Terra Preta de Índio–inspired amendments in a tropical latosol.
Autor:	LEITÃO, R. FERREIRA, T. FERREIRA, N. G. OROZCO-TERWENGEL, P. KILLE, P. BROWN, G. G. CUNHA, L.
Afiliación:	RICARDO LEITÃO, UNIVERSITY OF COIMBRA; TALITA FERREIRA, UNIVERSIDADE FEDERAL DO PARANÁ; NUNO G.C. FERREIRA, UNIVERSITY OF PORTO; PABLO OROZCO-TERWENGEL, CARDIFF UNIVERSITY; PETER KILLE, CARDIFF UNIVERSITY; GEORGE GARDNER BROWN, CNPF; LUÍS CUNHA, UNIVERSITY OF COIMBRA.
Año:	2026
Referencia:	Applied Soil Ecology, v. 225, 107278, 2026.
Descripción:	in tropical agroecosystems, inspired by the persistent fertility of Terra Preta de ´ Indio (TPI; Amazonian Dark Earth). Here, we evaluated the individual and combined effects of biochar, manure, and the earthworm Pontoscolex corethrurus on bacterial communities across bulk soil, maize roots, and earthworm gut in a factorial pot exper iment using an acidic, inherently nutrient-poor tropical Latosol as a model soil. High-throughput 16S rRNA gene sequencing revealed strong compartmentalisation of bacterial communities, while also identifying a prevalence- defined core shared across compartments that accounted for 75.4% of total sequence abundance despite limited taxonomic overlap. Responses to experimental factors were strongly compartment-dependent. In bulk soil, manure was the principal driver of bacterial community composition through shifts in pH and nutrient availability, whereas earthworms increased exchangeable Fe and restructured microbial networks toward greater modularity. Root- associated communities were comparatively buffered, whereas the earthworm gut remained responsive to manure, and biochar altered gut network topology. Crucially, the stacked TPI-inspired treatment (TPI mix), combining biochar, manure, earthworms, and maize, generated non-additive microbial trajectories and emergent community states across compartments. These microbial shifts were accompanied by improved functional out comes: while manure was the only individual factor to enhance maize growth, the stacked TPI mix produced the strongest overall response. Bradyrhizobium emerged as a key indicator taxon, reaching high relative abundance in both soil and earthworm gut under the integrated treatment. Our findings show that TPI-inspired amendment strategies can reorganise microbiomes across the soil–root–fauna continuum, with implications for soil biological functioning and fertility improvement in low-fertility soils.
Thesagro:	Animal Invertebrado Latossolo Solo Microrganismo
NAL Thesaurus:	Soil invertebrates Biochar Bradyrhizobium Earthworms Microbiome
Palabras clave:	Terra Preta de Índio Adubação orgânica Biocarvão
ISSN:	0929-1393
DOI:	https://doi.org/10.1016/j.apsoil.2026.107278
Tipo de Material:	Artigo de periódico
Acceso:	openAccess
Aparece en las colecciones:	Artigo em periódico indexado (CNPF)