Título:	Moxidectin residue in the serum of grazing meat lambs on three programs of endoparasites.
Autor:	FERNANDES, M. A. M. BAPTISTA, R. de C. GILAVERTE, S. PERES, M. T. P. ZAMONER, L. S. F. ASSALIN, M. R. MONTEIRO, A. L. G. REYES, F. G. R.
Afiliación:	MARIA ANGELA MACHADO FERNANDES, UNIVERSIDADE FEDERAL DO PARANÁ; RAFAELA DE CARVALHO BAPTISTA, UNIVERSIDADE FEDERAL DO PARANÁ; SUSANA GILAVERTE, UNIVERSIDADE FEDERAL DO PARANÁ; MYLENA TABORDA PIQUERA PERES, UNIVERSIDADE FEDERAL DO PARANÁ; LEONARDO STEFANO FERNANDES ZAMONER, UNIVERSIDADE FEDERAL DO PARANÁ; MARCIA REGINA ASSALIN, CNPMA; ALDA LÚCIA GOMES MONTEIRO, UNIVERSIDADE FEDERAL DO PARANÁ; FELIX GUILLERMO REYES REYES, UNIVERSIDADE ESTADUAL DE CAMPINAS.
Año:	2013
Referencia:	In: ANNUAL MEETING BRAZILIAN SOCIETY OF ANIMAL SCIENCE, 50., 2013, Campinas. The integration of Knowledge in animal production: abstracts. Campinas: SBZ, 2013. 1 CD ROM.
Descripción:	Abstract: The moxidectin residue in the serum of meat lambs produced on pastures and subjected to different programs of parasitic control was investigated. The experiment was performed at the Laboratory of Production and Research for Sheep and Goats (LAPOC), Federal University of Paraná (UFPR), Brazil, between January and May 2012. The lambs were kept with their dams on pasture of Tifton-85 (Cynodon spp.) until slaughter weight (30kg) and subjected to three programs of endoparasites control: (T1) regular deworming, every 28 days; (T2) deworming after the count of the number of eggs per gram of feces (EPG) if greater than 700 and (T3) selective deworming by the FAMACHA® method. The design was a randomized complete block with three treatments (control programs) and three replicates, with 14 lambs per program and 42 animals in total. Suffolk females and no castrated male lambs were homogeneously distributed on the blocks according to sex and type of birth (single and twin). Lambs presented initial weight and age of 19 kg and 42 days, respectively. Lambs were monitored for parasitic infection at intervals of 14 days after the starting of the evaluations (T2 e T3). The animals subjected to FAMACHA® guide (T3) that presented scores of 3, 4 or 5 were dewormed with anthelmintic drugs. At the regular control program (T1), all lambs were treated with the same drugs at the starting (day zero) and every 28 days, until slaughter. Regardless of the program for endoparasites control, the association of moxidectin (Cydectin®; 1 mL/50 kg body weight) with nitroxynil (Dovenix®; 1 mL/16.6 kg body weight) was used, because of proved efficacy for the flock. The recommended withdrawal period (28 days before slaughter) was respected for drugs dosing. The analysis to identify and quantify moxidectin residue levels in serum of lambs was performed at the Laboratory of Food Toxicology, University of Campinas (UNICAMP), Brazil. The validated analytical method presented between 80 to 107.3 % of accuracy and 1.7 to 6.7% of precision. This method was based on acetonitrile extraction, extract cleanup by dispersive solid phase using primary and secondary amine (PSA) plus octadecyl (C18), and analysis by liquid chromatography coupled with mass spectrometry (LC-ESI-MS/MS). The limits of detection and quantification were 2.0 ng.mL-1 and 5.0 ng.mL-1, respectively. The mean number of anthelmintic dosing per animal was 3.0 (T1), 2.9 (T2) and 2.7 (T3), ranging between 0 (none) and six per lamb. However, no moxidectin residue was detected on serum of lambs. Thus, the selective programs of endoparasite control (FAMACHA and EPG) or the anthelmintic application at regular intervals promoted no detectable moxidectin residue on serum of lambs, if respected the minimum withdrawal period of 28 days.
Thesagro:	Cordeiro Anti-Helmíntico Resíduo Método de Análise Análise Química
Tipo de Material:	Resumo em anais e proceedings
Acceso:	openAccess
Aparece en las colecciones:	Resumo em anais de congresso (CNPMA)