Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/13152
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dc.contributor.authorCASSIOLATO, A. M. R.eng
dc.contributor.authorMELO, I. S. deeng
dc.date.accessioned2019-07-18T00:44:14Z-
dc.date.available2019-07-18T00:44:14Z-
dc.date.created1999-09-08
dc.date.issued1999
dc.identifier.citationRevista de Microbiologia, v.30, p.43-46, 1999.eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/13152-
dc.descriptionThe isolation of genetic markers, like drug resistance and auxotrophy, is a laborious but important step in genetic research. The isolation of auxotrophic mutants of Trichoderma harzianum using the filtration enrichment technique was more effective than using the total isolation technique. Most of 12 auxotrophic mutants exhibited similar growth rate and higher sporulation when compared with the wild type, but only two mutants (TWS-410 and TW5-523) could grow in 500ug/L of benomyl.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectTechniqueseng
dc.subjectCulture mediumeng
dc.titleFiltration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.eng
dc.typeArtigo de periódicoeng
dc.date.updated2019-12-12T11:11:11Z
dc.subject.nalthesaurusbenomyleng
dc.subject.nalthesaurusbiological controleng
dc.subject.nalthesaurusfungieng
dc.subject.nalthesaurusmutantseng
dc.subject.nalthesaurusplant pathogenseng
dc.format.extent2p. 43-46eng
riaa.ainfo.id13152eng
riaa.ainfo.lastupdate2019-12-12 -02:00:00
dc.contributor.institutionEMBRAPA-CNPMA; ITAMAR SOARES DE MELO, CNPMA.eng
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