Title:	Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.
Authors:	CASSIOLATO, A. M. R. MELO, I. S. de
Affiliation:	EMBRAPA-CNPMA; ITAMAR SOARES DE MELO, CNPMA.
Date Issued:	1999
Citation:	Revista de Microbiologia, v.30, p.43-46, 1999.
Pages:	p. 43-46
Description:	The isolation of genetic markers, like drug resistance and auxotrophy, is a laborious but important step in genetic research. The isolation of auxotrophic mutants of Trichoderma harzianum using the filtration enrichment technique was more effective than using the total isolation technique. Most of 12 auxotrophic mutants exhibited similar growth rate and higher sporulation when compared with the wild type, but only two mutants (TWS-410 and TW5-523) could grow in 500ug/L of benomyl.
NAL Thesaurus:	benomyl biological control fungi mutants plant pathogens
Keywords:	Techniques Culture medium
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPMA)