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dc.contributor.authorFRAGOSO, R. da R.
dc.contributor.authorLOURENÇO, I. T.
dc.contributor.authorBATISTA, J. A. N.
dc.contributor.authorOLIVEIRA NETO, O. B.
dc.contributor.authorSILVA, M. C. M. da
dc.contributor.authorROCHA, T. L.
dc.contributor.authorCOUTINHO, M. V.
dc.contributor.authorSA, M. F. G. de
dc.date.accessioned2018-06-13T00:43:30Z-
dc.date.available2018-06-13T00:43:30Z-
dc.date.created2009-09-03
dc.date.issued2009
dc.identifier.citationExperimental Parasitology, v. 121, p. 115-123, 2009.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/257647-
dc.descriptionHerein we describe the cloning and characterization of a cDNA encoding an aspartic proteinase from the root-knot nematode Meloidogyne incognita. Using PCR techniques, a 1471-bp cDNA fragment encoding a cathepsin D-like (Mi-asp1) transcript was isolated from second-stage larvae mRNA. Its predicted amino acid sequence comprises a pro-region of 71 amino acid residues and a mature protease of 378 amino acid residues with a predicted molecular mass of 41.502 kDa. Protein sequence comparisons of Mi-asp1 with GenBank™ (DQ360827) sequences showed 59–71% identity with nematode-specific cathepsin D-like aspartic proteinases. Southern blot analysis, RT-PCR amplification and EST mining suggest the existence of a developmentally expressed gene family encoding aspartic proteinases in M. incognita. Mi-asp1 may represent a potential target for molecular intervention for the purposes of plant–parasitic nematode control.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleMeloidogyne incognita: molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase.
dc.typeArtigo de periódico
dc.date.updated2018-06-13T00:43:30Zpt_BR
dc.subject.nalthesaurusMeloidogyne
dc.subject.nalthesaurusMolecular cloningeng
dc.subject.nalthesaurusAspartic proteinaseseng
dc.subject.nalthesaurusCathepsin Deng
dc.subject.nalthesaurusRoot-knot nematodeseng
dc.subject.nalthesaurusCyst nematodeseng
dc.subject.nalthesaurusRapid amplification of cDNA endseng
dc.subject.nalthesaurusExpressed sequence tagseng
dc.subject.nalthesaurusReverse transcriptioneng
dc.subject.nalthesaurusPolymerase chain reactioneng
dc.subject.nalthesaurusOpen reading frameseng
riaa.ainfo.id257647
riaa.ainfo.lastupdate2018-06-12
dc.identifier.doihttps://doi.org/10.1016/j.exppara.2008.09.017eng
dc.contributor.institutionRODRIGO DA ROCHA FRAGOSO, CPAC
dc.contributor.institutionISABELA TRISTAN LOURENÇO, UNIVERSIDADE DE BRASÍLIAeng
dc.contributor.institutionJOÃO AGUIAR NOGUEIRA BATISTA, UNIVERSIDADE FEDERAL DE MINAS GERAISeng
dc.contributor.institutionOSMUNDO BRILHANTE OLIVEIRA NETOeng
dc.contributor.institutionMARIA CRISTINA MATTAR DA SILVA, CENARGENeng
dc.contributor.institutionTHALES LIMA ROCHA, CENARGENeng
dc.contributor.institutionMARISE VENTURA COUTINHO, CENARGENeng
dc.contributor.institutionMARIA FATIMA GROSSI DE SA, CENARGEN.eng
Appears in Collections:Artigo em periódico indexado (CENARGEN)

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