Title:	Meloidogyne incognita: molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase.
Authors:	FRAGOSO, R. da R. LOURENÇO, I. T. BATISTA, J. A. N. OLIVEIRA NETO, O. B. SILVA, M. C. M. da ROCHA, T. L. COUTINHO, M. V. SA, M. F. G. de
Affiliation:	RODRIGO DA ROCHA FRAGOSO, CPAC ISABELA TRISTAN LOURENÇO, UNIVERSIDADE DE BRASÍLIA JOÃO AGUIAR NOGUEIRA BATISTA, UNIVERSIDADE FEDERAL DE MINAS GERAIS OSMUNDO BRILHANTE OLIVEIRA NETO MARIA CRISTINA MATTAR DA SILVA, CENARGEN THALES LIMA ROCHA, CENARGEN MARISE VENTURA COUTINHO, CENARGEN MARIA FATIMA GROSSI DE SA, CENARGEN.
Date Issued:	2009
Citation:	Experimental Parasitology, v. 121, p. 115-123, 2009.
Description:	Herein we describe the cloning and characterization of a cDNA encoding an aspartic proteinase from the root-knot nematode Meloidogyne incognita. Using PCR techniques, a 1471-bp cDNA fragment encoding a cathepsin D-like (Mi-asp1) transcript was isolated from second-stage larvae mRNA. Its predicted amino acid sequence comprises a pro-region of 71 amino acid residues and a mature protease of 378 amino acid residues with a predicted molecular mass of 41.502 kDa. Protein sequence comparisons of Mi-asp1 with GenBank™ (DQ360827) sequences showed 59–71% identity with nematode-specific cathepsin D-like aspartic proteinases. Southern blot analysis, RT-PCR amplification and EST mining suggest the existence of a developmentally expressed gene family encoding aspartic proteinases in M. incognita. Mi-asp1 may represent a potential target for molecular intervention for the purposes of plant–parasitic nematode control.
NAL Thesaurus:	Meloidogyne Molecular cloning Aspartic proteinases Cathepsin D Root-knot nematodes Cyst nematodes Rapid amplification of cDNA ends Expressed sequence tags Reverse transcription Polymerase chain reaction Open reading frames
DOI:	https://doi.org/10.1016/j.exppara.2008.09.017
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CENARGEN)