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Title: Parameter evaluation in Michaelis-menten kinetics.
Authors: BRUNE, W.
Affiliation: EMBRAPA/CNPMS.
Date Issued: 1988
Citation: Arquivos de Biologia e Tecnologia, Curitiba, v. 31, n. 4, p. 527-538, 1988.
Description: Parameter estimation reliability in enzyme kinetics depends upon the substrate range concentrations under assay. An inappropriate concentration set may lead to spurious values of km and Vmax in the Michaelis-Menten approach. In this paper, the theoretical arguments for a practical criterium concerning the best work range of substrate concentration are discussed on a velocity ratio basis (V1/Vn) as response to the pertinent substrate concentration ratio (S1/Sn).
Thesagro: Química
Language: pt_BR
Type of Material: Artigo de periódico
Access: openAccess
Appears in Collections:Artigo em periódico indexado (CNPMS)

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