Title:	Parameter evaluation in Michaelis-menten kinetics.
Authors:	BRUNE, W. FABRIS, J. D.
Affiliation:	EMBRAPA/CNPMS.
Date Issued:	1988
Citation:	Arquivos de Biologia e Tecnologia, Curitiba, v. 31, n. 4, p. 527-538, 1988.
Description:	Parameter estimation reliability in enzyme kinetics depends upon the substrate range concentrations under assay. An inappropriate concentration set may lead to spurious values of km and Vmax in the Michaelis-Menten approach. In this paper, the theoretical arguments for a practical criterium concerning the best work range of substrate concentration are discussed on a velocity ratio basis (V1/Vn) as response to the pertinent substrate concentration ratio (S1/Sn).
Thesagro:	Química
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPMS)