Title:	Microsatellite markers in tropical legume (Centrosema pubescens Benth): development, characterization, and cross-species amplification in Centrosema sp.
Authors:	SOUSA, A. C. B. CARVALHO, M. A. BOAVENTURA, L. R. SFORÇA, D. A. CAMPOS, T. CANCADO, L. J. ZUCCHI, M. I. JANK, L. SOUZA, A. P.
Affiliation:	A. C. B. SOUSA, UNIVERSIDADE ESTADUAL DE CAMPINAS; MARCELO AYRES CARVALHO, CPAC; L. R. BOAVENTURA, UNIVERSIDADE ESTADUAL DE CAMPINAS; D. A. SFORÇA, UNIVERSIDADE ESTADUAL DE CAMPINAS; T. CAMPOS, UNIVERSIDADE ESTADUAL DE CAMPINAS; LETICIA JUNGMANN CANCADO, CNPGC; M. I. ZUCCHI, INSTITUTO AGRONÔMICO DE CAMPINAS; LIANA JANK, CNPGC; A. P. SOUZA, UNIVERSIDADE ESTADUAL DE CAMPINAS.
Date Issued:	2009
Citation:	Conservation Genetics Resources, v. 1, n. 1, p. 347-352, 2009.
Description:	Centrosema pubescens Benth is a forage legme widespread in tropical America. Twenty-six poly-morphic microsatellite markers were isolated and characterized in 15 genotypes of C. pubescens from the Cerrados Research Center Germsplams Bank of te Brazilian Agricultural Research Comporation (Embrapa). The number of alleles observed for each locus ranged from 2 to 5, with an average of 3 alleles per locus. The polymorphism information content (PIC) varied between 0.39 and 0.86 (average 0.57) and the discriminating power (D) ranged from 0.45 to 0.98 (average 0.68). The observed heterozygosity (Ho) and the expected heterozygosity (He) werw 0.01-0.81 and 0.10-0.86, respectively. A cross-amplification test in 11 Centrosema spécies suggest pottntial transferability of these microsatellites. The data indicated that the polymorphic microsatellite markers developed in this work should be useful for assessing genetic diversity in further breeding programs and germplasm conservation.
Thesagro:	Leguminosa tropical Marcador genético Genetica vegetal Variação Genética
DOI:	10.1007/s1268600990801
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPAC)