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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.contributor.author | STEINBERG, R. S. | |
| dc.contributor.author | PEREIRA, L. | |
| dc.contributor.author | LACORTE, G. A. | |
| dc.contributor.author | PEIXOTO, M. G. C. D. | |
| dc.contributor.author | VERNEQUE, R. da S. | |
| dc.contributor.author | TEODORO, R. L. | |
| dc.contributor.author | MACHADO, M. A. | |
| dc.contributor.author | FONSECA, C. G. | |
| dc.contributor.author | CARVALHO, M. R. S. | |
| dc.date.accessioned | 2024-02-16T11:32:30Z | - |
| dc.date.available | 2024-02-16T11:32:30Z | - |
| dc.date.created | 2010-04-26 | |
| dc.date.issued | 2009 | |
| dc.identifier.citation | Journal of Dairy Science, v. 92, n. 2, p. 773-776, 2009. | |
| dc.identifier.uri | http://www.alice.cnptia.embrapa.br/alice/handle/doc/711762 | - |
| dc.description | A new, quick, and inexpensive method for detecting the bovine acyl-CoA:diacylglycerol acyltransferase1 (DGAT1) polymorphism (K232A) through tetra-primer amplification refractory mutation system by PCR (ARMS-PCR) was developed in the present investigation. The DGAT1 gene was recently identified as underlying variation in milk production traits. To date, PCR techniques such as PCR-RFLP have been used for detecting the DGAT1 K232A polymorphism, despite being expensive and laborious. The method proposed here, a tetra-primer ARMS-PCR, showed 100% sensitivity and specificity when compared with PCR-RFLP results obtained in a sample of 80 animals tested in a double-blind system. Our results suggest that the use of tetra-primer ARMS-PCR for DGAT1 K232A polymorphism genotyping could greatly reduce costs providing information for both research purposes and for dairy cattle breeders who perform DGAT1 genotyping for gene-assisted selection. | |
| dc.language.iso | eng | |
| dc.rights | openAccess | |
| dc.subject | DGAT1 | |
| dc.title | Technical note: A new and cost-effective method for detection of the bovine acyl-CoA: diacyglycerol acyltransferase 1 K232A polymorphism in cattle. | |
| dc.type | Artigo de periódico | |
| riaa.ainfo.id | 711762 | |
| riaa.ainfo.lastupdate | 2024-02-16 | |
| dc.contributor.institution | R. S. STEINBERG, UFMG; L. PEREIRA, UFMG; G. A. LACORTE, UFMG; MARIA GABRIELA CAMPOLINA D PEIXOTO, CNPGL; RUI DA SILVA VERNEQUE, CNPGL; ROBERTO LUIZ TEODORO, Pesquisador aposentado do CNPGL; MARCO ANTONIO MACHADO, CNPGL; C. G. FONSECA, UFMG; M. R. S. CARVALHO, UFMG. | |
| Aparece en las colecciones: | Nota Técnica/Nota científica (CNPGL)  | |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| Technical-note-A-new-and-cost-effective-method-for-detection-of-the-bovine-acyl-CoA.pdf | 271.92 kB | Adobe PDF |  Visualizar/Abrir |
