Title:	Technical note: A new and cost-effective method for detection of the bovine acyl-CoA: diacylglycerol acyltransferase 1 K232A polymorphism in cattle.
Authors:	STEINBERG, R. S. PEREIRA, L. LACORTE, G. A. PEIXOTO, M. G. C. D. VERNEQUE, R. da S. TEODORO, R. L. MACHADO, M. A. FONSECA, C. G. CARVALHO, M. R. S.
Affiliation:	R. S. STEINBERG, UFMG; L. PEREIRA, UFMG; G. A. LACORTE, UFMG; MARIA GABRIELA CAMPOLINA D PEIXOTO, CNPGL; RUI DA SILVA VERNEQUE, CNPGL; ROBERTO LUIZ TEODORO, Pesquisador aposentado do CNPGL; MARCO ANTONIO MACHADO, CNPGL; C. G. FONSECA, UFMG; M. R. S. CARVALHO, UFMG.
Date Issued:	2009
Citation:	Journal of Dairy Science, v. 92, n. 2, p. 773-776, 2009.
Description:	A new, quick, and inexpensive method for detecting the bovine acyl-CoA:diacylglycerol acyltransferase1 (DGAT1) polymorphism (K232A) through tetra-primer amplification refractory mutation system by PCR (ARMS-PCR) was developed in the present investigation. The DGAT1 gene was recently identified as underlying variation in milk production traits. To date, PCR techniques such as PCR-RFLP have been used for detecting the DGAT1 K232A polymorphism, despite being expensive and laborious. The method proposed here, a tetra-primer ARMS-PCR, showed 100% sensitivity and specificity when compared with PCR-RFLP results obtained in a sample of 80 animals tested in a double-blind system. Our results suggest that the use of tetra-primer ARMS-PCR for DGAT1 K232A polymorphism genotyping could greatly reduce costs providing information for both research purposes and for dairy cattle breeders who perform DGAT1 genotyping for gene-assisted selection.
Keywords:	DGAT1
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Nota Técnica/Nota científica (CNPGL)