Título:	Garlic viral complex: identification of Potyviruses and Carlavirus in central Brazil.
Autoria:	FAJARDO, T. V. M. NISHIJIMA, M. BUSO, J. A. TORRES, A. C. AVILA, A. C. RESENDE, R. O.
Afiliação:	EMBRAPA-CNPH, Brasilia, DF.
Ano de publicação:	2001
Referência:	Fitopatologia Brasileira, Brasilia, v.26, n.3, p.619-626, set. 2001.
Conteúdo:	Garlic viruses often occur in complex infections in nature. In this study, a garlic virus complex, collected in fields in Brazil, was purified. RT-PCR was performed using specific primers designed from the consensus regions of the coat protein genes of Onion yellow dwarf virus, a garlic strain (OYDV-G) and Leek yellow stripe virus (LYSV). cDNA of Garlic common latent virus (GCLV) was synthesized using oligo-dT and random primers. By these procedures individual garlic virus genomes were isolated and sequenced. The nucleotide sequence analysis associated with serological data reveals the presence of two Potyvirus OYDV-G and LYSV, and GCLV, a Carlavirus, simultaneously infecting garlic plants. Deduced amino acid sequences of the Brazilian isolates were compared with related viruses reported in different geographical regions of the world. The analysis showed closed relations considering the Brazilian isolates of OYDV-G and GCLV, and large divergence considering LYSV isolate. The detection of these virus species was confirmed by specific reactions observed when coat protein genes of the Brazilian isolates were used as probes in dot-blot and Southern blot hybridization assays. In field natural viral re-infection of virus-free garlic was evaluated.
Thesagro:	Alho Allium Sativum Cerrado Doença Identificação Vírus
NAL Thesaurus:	Brazil
Palavras-chave:	Complexo viral Regiao Centro Oeste Garlic Diseases Viral complex OYDV-G LYSV GCLV Identification
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPH)