Title:	Expression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae).
Authors:	LOPES, M. A. HORA JUNIOR, B. T. DIAS, C. V. SANTOS, G. C. GRAMACHO, K. P. CASCARDO, J. C. M. GESTEIRA, A. da S. MICHELI, F.
Affiliation:	Karina Peres Gramacho, CEPLAC; Júlio Cézar Mattos Cascardo, UESC; ABELMON DA SILVA GESTEIRA, CNPMF; Fabienne Micheli, UESC.
Date Issued:	2010
Citation:	Genetics and Molecualr Reseach, Ribeirão Preto, v. 9, n. 3, p. 1279-1297, 2010.
Description:	Cacao (Theobroma cacao) is one of the most important tropical crops; however, production is threatened by numerous pathogens, including the hemibiotrophic fungus Moniliophthora perniciosa, which causes witches? broom disease. To understand the mechanisms that lead to the development of this disease in cacao, we focused our attention on cacao transcription factors (TFs), which act as master regulators of cellular processes and are important for the fine-tuning of plant defense responses. We developed a macroarray with 88 TF cDNA from previously obtained cacao-M. perniciosa interaction libraries. Seventy-two TFs were found differentially expressed between the susceptible (Catongo) and resistant (TSH1188) genotypes and/or during the disease time course - from 24 h to 30 days after infection. Most of the differentially expressed TFs belonged to the bZIP, MYB and WRKY families and presented opposite expression patterns in susceptible and resistant cacao-M. perniciosa interactions (i.e., up-regulated in Catongo and down-regulated in TSH1188). The results of the macroarray were confirmed for bZIP and WRKY TFs by real-time PCR. These differentially expressed TFs are good candidates for subsequent functional analysis as well as for plant engineering. Some of these TFs could also be localized on the cacao reference map related to witches? broom resistance, facilitating the breeding and selection of resistant cacao trees.
Thesagro:	Cacau Doença de Planta Fungo Genótipo Hibridação Vassoura de Bruxa
Keywords:	Macroarrays RT-qPCR Gene regulation Theobrama cacao Witches broom disease
ISSN:	1676-5680
DOI:	10.4238/vol9-3gmr825
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPMF)