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dc.contributor.authorCUNHA, E. R.
dc.contributor.authorSILVA, C. G.
dc.contributor.authorSIQUEIRA, L. G. B.
dc.contributor.authorMARTINS, C. F.
dc.date.accessioned2026-04-15T17:54:07Z-
dc.date.available2026-04-15T17:54:07Z-
dc.date.created2010-11-19
dc.date.issued2010
dc.identifier.citationAnimal Reproduction, v. 7, n. 3, p. 317, Jul./Sept. 2010.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/867462-
dc.descriptionAdult somatic cells can undergo nuclear reprogramming after fusion with a mature oocyte, a process known as somatic cell nuclear transfer or cloning. An important requisite is the availability of cells able of undergoing reprogramming to support embryonic and fetal development after nuclear transfer. In this context, the bovine amniotic cells, which are relatively less differentiated, have a potential for use in nuclear transfer. Thus, this study seeking alternatives to improve the efficiency of bovine nuclear transfer aimed to evaluate the possibility of isolation and storage of amniotic cells, comparing the efficiency of three cryoprotectant solutions.
dc.language.isoeng
dc.rightsopenAccess
dc.titleIsolation, culture and cryopreservation of bovine amniotic cells with Dimethyl sulfoxide, Dimethylformamide or Glycerol.
dc.typeArtigo de periódico
dc.subject.thesagroBovino
dc.subject.thesagroCriopreservação
dc.subject.nalthesaurusCryopreservation
dc.subject.nalthesaurusDimethyl sulfoxide
dc.subject.nalthesaurusDimethylformamide
dc.subject.nalthesaurusGlycerol
dc.description.notesEdição dos resumos do III International Symposium Animal Biology of Reproductive, 3., 2010, Águas de São Pedro.
riaa.ainfo.id867462
riaa.ainfo.lastupdate2026-04-15
dc.contributor.institutionLUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; CARLOS FREDERICO MARTINS, CPAC.
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