Title:	Isolation, culture and cryopreservation of bovine amniotic cells with Dimethyl sulfoxide, Dimethylformamide or Glycerol.
Authors:	CUNHA, E. R. SILVA, C. G. SIQUEIRA, L. G. B. MARTINS, C. F.
Affiliation:	LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; CARLOS FREDERICO MARTINS, CPAC.
Date Issued:	2010
Citation:	Animal Reproduction, v. 7, n. 3, p. 317, Jul./Sept. 2010.
Description:	Adult somatic cells can undergo nuclear reprogramming after fusion with a mature oocyte, a process known as somatic cell nuclear transfer or cloning. An important requisite is the availability of cells able of undergoing reprogramming to support embryonic and fetal development after nuclear transfer. In this context, the bovine amniotic cells, which are relatively less differentiated, have a potential for use in nuclear transfer. Thus, this study seeking alternatives to improve the efficiency of bovine nuclear transfer aimed to evaluate the possibility of isolation and storage of amniotic cells, comparing the efficiency of three cryoprotectant solutions.
Thesagro:	Bovino Criopreservação
NAL Thesaurus:	Cryopreservation Dimethyl sulfoxide Dimethylformamide Glycerol
Notes:	Edição dos resumos do III International Symposium Animal Biology of Reproductive, 3., 2010, Águas de São Pedro.
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPAC)