Title:	Detection of coffee ringspot virus (CoRSV) in the mite vector by RT-qPCR.
Authors:	COSTA, F. M. BASTIANEL, M. FREITAS-ASTUA, J. KUBO, K. S. MACHADO, M. A. GONDIM JUNIOR, M. G. C.
Affiliation:	F. M. Costa, UFRPE; Marinês Bastianel, APTA; JULIANA DE FREITAS ASTUA, CNPMF; Karen Sumire Kubo, IAC; Marcos Antonio Machado, APTA; Manoel Guedes Corrêa Gondim Júnior, APTA.
Date Issued:	2010
Citation:	In:INTERNATIONAL CONGRESS OF ACAROLOGY, 13., 2010, Recife. Abstracts book... Recife: CNPq: FACEPE: Banco do Nordeste: TWAS, 2010. p. 61-62.
Description:	The mite Brevipalpus phoenicis (Geijskes, 1939) (Acari: Tenuipalpidae) is responsible for the transmission of coffee ringspot. There is a great interest in the study of Brevipalpus transmitted viruses (BrTVs), and the interaction between them, their vectors and hosts. Electron microscopy analyses suggest that CoRSV replicates in the mite tissue, what would characterize a persistent propagative virus-vector relationship. This study aimed to establish a transcriptase quantitative polymerase chain reaction (RT-qPCR) method to evaluate and compare the replication capacity of the virus in the vector. Total RNA was extracted from a sample of 100 mites reared in symptomatic plants for CoRSV using two different methods: CTAB and Nucleo Spin RNA XS Kit (Macherey-Nagel) and quantified in a NanoDrop 8000 (Thermo Scientific).
Thesagro:	Brevipalpus Phoenicis Vírus
Notes:	Abstract 109
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (CNPMF)