Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/902616
Research center of Embrapa/Collection: Embrapa Café - Artigo em periódico indexado (ALICE)
Date Issued: 2011
Type of Material: Artigo em periódico indexado (ALICE)
Authors: REZENDE, J. C. de
CARVALHO, C. H. S. de
PASQUAL, M.
SANTOS, A. C. R.
CARVALHO, S. M. de
Additional Information: EPAMIG; CARLOS HENRIQUE S DE CARVALHO, SAPC; Universidade Federal de Lavras; CEIS.
Title: Calli induction in leaf explants of coffee elite genotypes.
Publisher: Ciência Rural, Santa Maria, v.41, n.3, p. 384-389, mar, 2011.
Language: en
Keywords: Growh regulators.
Description: Three experiments were carried out with the objective of achieving high effectiveness in calli induction from high heterozygosis leaf explants of Coffea arabica through indirect somatic embryogenesis. A randomized-block design in a 2x5 factorial arrangement made up of two media [BOXTEL & BERTHOULY (1996) and TEIXEIRA et al. (2004)] and five C. arabica genotypes were used in the first experiment. In the second experiment the embryogenic calli production potential was evaluated in ten genotypes. Each of them was considered as a treatment. In the third experiment the variations in both 2.4-D (2.5 e 20μM) and 2-iP (2.5 e 20μM) concentrations in TEIXEIRA et al. (2004) medium and secondary media were evaluated. Crops were kept in a growth room under darkness, at 25±2oC. The medium described by TEIXEIRA et al (2004) was found to be superior when compared to that described by BOXTEL & BERTHOULY (1996) in the 2.2 and 7.2 genotypes. An opposite behavior was noticed in 4.2 genotype, that is, BOXTEL & BERTHOULY (1996) had medium superiority. Both 3.0 and 5.0 genotypes had the same behavior in both media studied, which shows that the somatic embryo production depends on the genotype. Calli induction depends on the 2-iP and 2.4 D ratio. The 20.0μM of 2.4-D and 20.0μM of 2-iP combination caused the highest embryogenic calli induction rate.
Thesagro: Coffea Arábica.
Data Created: 2011-10-06
Appears in Collections:Artigo em periódico indexado (SAPC)

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