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dc.contributor.authorCARVALHO, F. S.
dc.contributor.authorWENCESLAU, A. A.
dc.contributor.authorTEIXEIRA, M.
dc.contributor.authorALBUQUERQUE, G. R.
dc.date.accessioned2018-01-26T23:36:59Z-
dc.date.available2018-01-26T23:36:59Z-
dc.date.created2011-12-02
dc.date.issued2011
dc.identifier.citationGenetics and Molecular Research, v. 10, n. 2, p. 996-1005, 2011.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/908376-
dc.descriptionAbstract - We used PCR to test various protocols and define a technique for DNA extraction directly from chicken-shed stool samples for the identification of Eimeria species that parasitize birds. It was possible to extract and amplify DNA of seven Eimeria species from field stool samples, using both protocols tested; extractions made with phenol/chloroform protocols gave the best results. The primers were specific and sensitive, allowing amplification of samples containing as few as 20 oocysts, both in individual and in a multiplex PCR. Individualized PCR with the phenol/chloroform DNA extraction protocol detected a larger number of Eimeria species. Molecular diagnosis was found to be practical and precise, and can be used for monitoring and epidemiological studies of Eimeria.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPCR
dc.subjectReação em cadeia
dc.subjectPolimerasept_BR
dc.subjectMultiplex PCRpt_BR
dc.subjectBroilerspt_BR
dc.subjectEimeriosispt_BR
dc.subjectPrevalênciapt_BR
dc.titleMolecular diagnosis of Eimeria species affecting naturally infected Gallus gallus.
dc.typeArtigo de periódico
dc.date.updated2019-09-23T11:11:11Zpt_BR
dc.subject.thesagroFrango
dc.subject.nalthesaurusEimeriapt_BR
riaa.ainfo.id908376
riaa.ainfo.lastupdate2019-09-23 -03:00:00
dc.identifier.doi10.4238/vol10-2gmr1043
dc.contributor.institutionF.S. Carvalho, Universidade Estadual de Santa Cruz - Ilhéus, BA.; A.A. Wenceslau, Universidade Estadual de Santa Cruz - Ilhéus, BA; MARCEL TEIXEIRA, CNPC; G.R. Albuquerque, Universidade Estadual de Santa Cruz - Ilhéus, BA.
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