Título:	Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus.
Autoria:	CARVALHO, F. S. WENCESLAU, A. A. TEIXEIRA, M. ALBUQUERQUE, G. R.
Afiliação:	F.S. Carvalho, Universidade Estadual de Santa Cruz - Ilhéus, BA.; A.A. Wenceslau, Universidade Estadual de Santa Cruz - Ilhéus, BA; MARCEL TEIXEIRA, CNPC; G.R. Albuquerque, Universidade Estadual de Santa Cruz - Ilhéus, BA.
Ano de publicação:	2011
Referência:	Genetics and Molecular Research, v. 10, n. 2, p. 996-1005, 2011.
Conteúdo:	Abstract - We used PCR to test various protocols and define a technique for DNA extraction directly from chicken-shed stool samples for the identification of Eimeria species that parasitize birds. It was possible to extract and amplify DNA of seven Eimeria species from field stool samples, using both protocols tested; extractions made with phenol/chloroform protocols gave the best results. The primers were specific and sensitive, allowing amplification of samples containing as few as 20 oocysts, both in individual and in a multiplex PCR. Individualized PCR with the phenol/chloroform DNA extraction protocol detected a larger number of Eimeria species. Molecular diagnosis was found to be practical and precise, and can be used for monitoring and epidemiological studies of Eimeria.
Thesagro:	Frango
NAL Thesaurus:	Eimeria
Palavras-chave:	PCR Reação em cadeia Polimerase Multiplex PCR Broilers Eimeriosis Prevalência
Digital Object Identifier:	10.4238/vol10-2gmr1043
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPC)