Título:	Single primer-based DNA amplification as a suitable and low-cost tool for assessing genetic diversity in mangrove crabs.
Autoria:	BRITTO, F. B. D. S. F. MENDES OGAWA, M. CINTRA, I. H. A. DINIZ, F. M.
Afiliação:	FABIO BARROS BRITTO, UFPI; MENDES, D. S. F., CPAMN; M. OGAWA, UFCE; ISRAEL HIDENBURGO ANICETO CINTRA, UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA; FABIO MENDONCA DINIZ, CPAMN.
Ano de publicação:	2011
Referência:	Genetics and Molecular Research, Ribeirão Preto, v. 10, n. 4, p. 4084-4092, 2011.
Conteúdo:	We used single primer-based DNA markers to assess genetic variability of the mangrove crab, Ucides cordatus, collected from four different localities from Pará to Santa Catarina States in Brazil (almost 5000 km distant). Five primers were chosen based on the consistency of the amplified bands and the polymorphism of each locus. A total of 78 loci were amplified in 76 samples; high polymorphism rates were detected in the entire sample (80.8%) and within each locality (73.5-79.5%). Analysis of molecular variance demonstrates significant differences between localities (P < 0.001); however, the &#934;ST value (0.078) indicates a low level of genetic differentiation, which suggests that U. cordatus larvae can spread over large distances. The variation was distributed among the samples, and most of it was attributed to differences among individuals within localities. Cluster analysis, based on the Jaccard similarity coefficient, and the Mantel test gave similar results to the analysis of molecular variance data. Despite the low level of population structuring, these markers could be used for studying U. cordatus diversity, due to the high level of polymorphism.
Thesagro:	Caranguejo Marcador Molecular
Palavras-chave:	Diversidade
ISSN:	1676-5680
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPAMN)