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dc.contributor.authorSILVA, G. A. dapt_BR
dc.contributor.authorBERNARDI, T. L.pt_BR
dc.contributor.authorSCHAKER, P. D. C.pt_BR
dc.contributor.authorMENEGOTTO, M.pt_BR
dc.contributor.authorVALENTE, P.pt_BR
dc.date.accessioned2012-05-22T11:11:11Zpt_BR
dc.date.accessioned2012-05-22T11:11:11Zpt_BR
dc.date.available2012-05-22T11:11:11Zpt_BR
dc.date.available2012-05-22T11:11:11Zpt_BR
dc.date.created2012-05-22pt_BR
dc.date.issued2012pt_BR
dc.identifier.citationBrazilian Archives of Biology and Technology, Curitiba, v. 55, n. 2, p. 319-327, mar./abr. 2012.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/925175pt_BR
dc.descriptionThe purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisiae was chosen. The described boiling and freeze-thawing protocols generated the PCR-grade product preparations and could be used to process many samples. The amplification of the fragments could be observed after 30 and 35 cycles. These processes of extraction without using any kind of chemical reagents, especial water, and purification procedures proved to be efficient, reproducible, simple, fast, and inexpensive.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectGelo-degelopt_BR
dc.subjectEbuliçãopt_BR
dc.subjectExtração de DNApt_BR
dc.subjectMetodologiapt_BR
dc.titleRapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2012-05-22T11:11:11Zpt_BR
dc.subject.thesagroMicrobiologiapt_BR
dc.subject.thesagroDNApt_BR
dc.subject.thesagroSaccharomyces Cerevisiaept_BR
dc.subject.nalthesaurusDekkera bruxellensispt_BR
riaa.ainfo.id925175pt_BR
riaa.ainfo.lastupdate2012-05-22pt_BR
dc.contributor.institutionGILDO ALMEIDA DA SILVA, CNPUV; Taís Letícia Bernardi, CNPUV (BOLSISTA); Patrícia Dayane Carvalho Schaker, CNPUV (BOLSISTA); Morgana Menegotto, CNPUV (BOLSISTA); Patricia Valente, UFRGS.pt_BR
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