Title:	Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification.
Authors:	SILVA, G. A. da BERNARDI, T. L. SCHAKER, P. D. C. MENEGOTTO, M. VALENTE, P.
Affiliation:	GILDO ALMEIDA DA SILVA, CNPUV; Taís Letícia Bernardi, CNPUV (BOLSISTA); Patrícia Dayane Carvalho Schaker, CNPUV (BOLSISTA); Morgana Menegotto, CNPUV (BOLSISTA); Patricia Valente, UFRGS.
Date Issued:	2012
Citation:	Brazilian Archives of Biology and Technology, Curitiba, v. 55, n. 2, p. 319-327, mar./abr. 2012.
Description:	The purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisiae was chosen. The described boiling and freeze-thawing protocols generated the PCR-grade product preparations and could be used to process many samples. The amplification of the fragments could be observed after 30 and 35 cycles. These processes of extraction without using any kind of chemical reagents, especial water, and purification procedures proved to be efficient, reproducible, simple, fast, and inexpensive.
Thesagro:	Microbiologia DNA Saccharomyces Cerevisiae
NAL Thesaurus:	Dekkera bruxellensis
Keywords:	Gelo-degelo Ebulição Extração de DNA Metodologia
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPUV)