Title:	Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil.
Authors:	FRANÇA, C. A. PEIXOTO, R. M. CAVALCANTE, M. B. MELO, N. F. de OLIVEIRA, C. J. B. VESCHI, J. L. A. MOTA, R. A. COSTA, M. M.
Affiliation:	CHIRLES A. FRANÇA; RODOLFO M. PEIXOTO, Instituto Federal de Educação, Ciência e Tecnologia do Sertão Pernambucano, Campus Floresta; MARIELLY B. CAVALCANTE; NATONIEL FRANKLIN DE MELO, CPATSA; CELSO JOSÉ B. OLIVEIRA, UFPB; JOSIR LAINE APARECIDA VESCHI, CPATSA; RINALDO A. MOTA, UFRPE; MATEUS M. COSTA, UNIVASF.
Date Issued:	2012
Citation:	Pesquisa Veterinária Brasileira, v. 32, n. 8, p. 747-753, ago. 2012.
Description:	The study aimed to determine the antimicrobial resistance patterns and to identify molecular resistance markers in Staphylococcus spp. (n=210) isolated from small ruminant mastitis in Brazil. The antimicrobial resistance patterns were evaluated by the disk diffusion test and by detection of the presence of mecA, blaZ, ermA, ermB, ermC and msrA genes by PCR. The efflux pump test was performed using ethidium bromide and biofilm production was determined by Congo red agar test along with PCR for detection of the icaD gene. The isolates were most resistant to amoxicillin (50.0%), streptomycin (42.8%), tetracycline (40.4%), lincomycin (39.0%) and erythromycin (33.8%). Pan-sus- ceptibility to all tested drugs was observed in 71 (33.8%) isolates and 41 Staphylococcus isolates were positive for the efflux pump. Although phenotypic resistance to oxacillin was observed in 12.8% of the isolates, none harbored the mecA gene. However, 45.7% of the isolates harbored blaZ indicating that beta-lactamase production was the main mechanism associated with staphylococci resistance to beta-lactams in the present study. The other determinants of resistance to antimicrobial agents ermA, ermB, ermC, and msrA were observed in 1.4%, 10.4%, 16.2%, and 0.9% of the isolates, respectively. In addition, the icaD gen was detected in 32.9% of the isolates. Seventy three isolates (54 from goats and 19 from sheep) were negative for all resistance genes tested and 69 isola - tes presented two or more resistance genes. Association among blaZ, ermA, ermB, ermC and efflux pump were observed in 17 isolates, 14 of which originated from goats and three from sheep. The data obtained in this study show the resistance of the isolates to beta-lactamics, which may be associated with the use of antimicrobial drugs without veterinary control.
Thesagro:	Caprino Ovino Biofilme Cabra Leite
NAL Thesaurus:	Goats Sheep Staphylococcus
Keywords:	Bomba de efluxo Mastite
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPATSA)