Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/960405
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dc.contributor.authorDUBIELA, C. R.pt_BR
dc.contributor.authorFAJARDO, T. V. M.pt_BR
dc.contributor.authorSOUTO, E. R.pt_BR
dc.contributor.authorNICKEL, O.pt_BR
dc.contributor.authorEIRAS, M.pt_BR
dc.contributor.authorREVERS, L. F.pt_BR
dc.date.accessioned2014-02-04T22:23:31Z-
dc.date.available2014-02-04T22:23:31Z-
dc.date.created2013-06-21pt_BR
dc.date.issued2013pt_BR
dc.identifier.citationTropical Plant Pathology, Viçosa, v. 38, n. 2, p. 158-165, mar./apr. 2013pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/960405pt_BR
dc.descriptionThe aim of this work was to evaluate the efficiency of real-time RT-PCR for detection of different isolates of ten important virus species that infect grapevines in Brazil: Grapevine leafroll-associated virus (GLRaV-1, -2, -3 and -5), Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine virus D (GVD), Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine fleck virus (GFkV) and Grapevine fanleaf virus (GFLV). The reactions consisted of individual (simplex) and simultaneous (duplex) virus detections. Thirty six grapevine accessions, regenerated after thermotherapy and tissue culture treatments, have been analysed. All the above-mentioned viruses were sensitively detected in simplex reactions in samples infected with different virus isolates. Specifically to GLRaV-1 it was necessary to mix reagents refered by different sources to achieve the amplification. GVA, GRSPaV, GLRaV-2 and GLRaV-3 combined with GVB, GFLV, GFkV, GVD and GLRaV-5 were accurately detected in duplex trials. It was shown, that real-time RT-PCR (TaqMan) is able to efficiently detect different local virus species and isolates.pt_BR
dc.language.isoporpt_BR
dc.rightsopenAccesspt_BR
dc.titleSimultaneous detection of Brazilian isolates of grapevine viruses by TaqMan real-time RT-PCR.pt_BR
dc.typeArtigo em anais e proceedingspt_BR
dc.date.updated2019-04-12T11:11:11Zpt_BR
dc.subject.thesagroViticulturapt_BR
dc.subject.thesagroUvapt_BR
dc.subject.thesagroDoença de plantapt_BR
dc.subject.thesagroVíruspt_BR
dc.subject.thesagroDiagnosticopt_BR
dc.subject.nalthesaurusClosteroviridaept_BR
dc.subject.nalthesaurusBetaflexiviridaept_BR
dc.description.notesComunicação científica.pt_BR
riaa.ainfo.id960405pt_BR
riaa.ainfo.lastupdate2019-04-12 -03:00:00pt_BR
dc.contributor.institutionCARLA R. DUBIELA, UEM; THOR VINICIUS MARTINS FAJARDO, CNPUV; ELIEZER R. SOUTO, UEM; OSMAR NICKEL, CNPUV; MARCELO EIRAS, INSTITUTO BIOLÓGICO; LUIS FERNANDO REVERS, CNPUV.pt_BR
Appears in Collections:Artigo em anais de congresso (CNPUV)

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