Title:	Simultaneous detection of Brazilian isolates of grapevine viruses by TaqMan real-time RT-PCR.
Authors:	DUBIELA, C. R. FAJARDO, T. V. M. SOUTO, E. R. NICKEL, O. EIRAS, M. REVERS, L. F.
Affiliation:	CARLA R. DUBIELA, UEM; THOR VINICIUS MARTINS FAJARDO, CNPUV; ELIEZER R. SOUTO, UEM; OSMAR NICKEL, CNPUV; MARCELO EIRAS, INSTITUTO BIOLÓGICO; LUIS FERNANDO REVERS, CNPUV.
Date Issued:	2013
Citation:	Tropical Plant Pathology, Viçosa, v. 38, n. 2, p. 158-165, mar./apr. 2013
Description:	The aim of this work was to evaluate the efficiency of real-time RT-PCR for detection of different isolates of ten important virus species that infect grapevines in Brazil: Grapevine leafroll-associated virus (GLRaV-1, -2, -3 and -5), Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine virus D (GVD), Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine fleck virus (GFkV) and Grapevine fanleaf virus (GFLV). The reactions consisted of individual (simplex) and simultaneous (duplex) virus detections. Thirty six grapevine accessions, regenerated after thermotherapy and tissue culture treatments, have been analysed. All the above-mentioned viruses were sensitively detected in simplex reactions in samples infected with different virus isolates. Specifically to GLRaV-1 it was necessary to mix reagents refered by different sources to achieve the amplification. GVA, GRSPaV, GLRaV-2 and GLRaV-3 combined with GVB, GFLV, GFkV, GVD and GLRaV-5 were accurately detected in duplex trials. It was shown, that real-time RT-PCR (TaqMan) is able to efficiently detect different local virus species and isolates.
Thesagro:	Viticultura Uva Doença de planta Vírus Diagnostico
NAL Thesaurus:	Closteroviridae Betaflexiviridae
Notes:	Comunicação científica.
Type of Material:	Artigo em anais e proceedings
Access:	openAccess
Appears in Collections:	Artigo em anais de congresso (CNPUV)