Title:	Genetic variability of elite barley genotypes for brazilian savanna irrigated systems based on RAPD markets.
Authors:	AMABILE, R. F. FALEIRO, F. G. CAPETTINI, F. RIBEIRO JUNIOR, W. Q. PEIXOTO, J. R. ALMEIDA, B. C. de
Affiliation:	RENATO FERNANDO AMABILE, CPAC FABIO GELAPE FALEIRO, CPAC FLÁVIO CAPETTINI, ALBERTA AGRICULTURE AND RURAL DEVELOPMENT WALTER QUADROS RIBEIRO JUNIOR, CPAC JOSÉ RICARDO PEIXOTO, UNIVERSIDADE DE BRASÍLIA BERNARDO COUTINHO DE ALMEIDA, UNIVERSIDADE FEDERAL DO TOCANTINS.
Date Issued:	2014
Citation:	Bioscience Journal, Uberlândia, v. 30, n. 4, p. 1118-1126, jul./ago. 2014.
Pages:	p. 1118-1126
Description:	The objective of this work was to characterize and quantify the genetic variability of 39 barley elite genotypes from a Brazilian working collection belonging to Embrapa, using RAPD (Random Amplified Polymorphic DNA) molecular markers. Genomic DNA samples were extracted from leaves of each genotype and 15 decamer primers were used to obtain RAPD molecular markers. Molecular markers were converted in a binary data matrix utilized to estimate genetic dissimilarities between genotypes and to realize grouping and dispersion graphic analysis. A total of 160 RAPD markers were obtained, making 10.7 markers medium per primer. From all the markers, 141 (88.12%) were polymorphic. Genetic dissimilarities varied from 0.049 to 0.337 among the genotypes. PFC 2004033 and Prestige cultivar showed biggest genetic dissimilarities to others genetic materials. Grouping and dispersion graphic analysis showed a clustering tendency between the Mexican and American genotypes. Another clustering tendency was also found concerning the six-rowed materials. Accessions developed and used in Brazil and also in Germany, UK and Australia have shown the greatest genetic dissimilarity among themselves, being considered promising options to increase the genetic base of breeding programs.
Thesagro:	Cevada Variação genética Marcador genético Recurso genético Cerrado Hordeum Vulgare
NAL Thesaurus:	Barley Genetic variation Genetic markers Genetic resources Savannas Random amplified polymorphic DNA technique
Notes:	Título em português: Variabilidade genética de acessos elite de cevada para sistemas irrigados no Cerrado com base em marcadores RAPD.
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPAC)