Título:	Stress tolerance in peanuts: a genomic approach using wild Arachis
Autor:	BRASILEIRO, A. C. M. MORGANTE, C. V. ARAUJO, A. C. G. de GUIMARÃES, L. A. BERTIOLI, S. C. de M. L. BERTIOLI, D. FONSECA, L. N. SARAIVA, M. A. de P. MARTINS, A. C. A GUIMARAES, P. M.
Afiliación:	ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN; CAROLINA VIANNA MORGANTE, CPATSA; ANA CLAUDIA GUERRA DE ARAUJO, CENARGEN; SORAYA CRISTINA DE M LEAL BERTIOLI, CENARGEN; D. BERTIOLI, UnB; LEONARDO NUNES FONSECA, CENARGEN; MARIO ALFREDO DE PASSOS SARAIVA, CENARGEN; A. C. Q. MARTINS; PATRICIA MESSEMBERG GUIMARAES, CENARGEN.
Año:	2013
Referencia:	In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilheus. The challenge for the 21st century: book of abstracts. [S.l.]: International Advanced Biology Consortium, 2013.
Páginas:	p. 14.
Descripción:	Cultivated peanut (Arachis hypogaea) has a high morphological but narrow genetic diversity, and understanding the genetic processes of this plant is hindered by the fact that the peanut genome has not yet been sequenced and its genomic resources are still limited. Due to its high genetic diversity and adaptation to a range of environments throughout the evolution course, wild relatives of peanut (Arachis spp.) constitute a rich source of allele diversity for resistance to abiotic and biotic stresses. In particular, A. duranensis and A. stenosperma harbor high adaptability to water stress conditions and root-knot nematode resistance, respectively. In order to identify genes in those wild species that are differentially expressed in response to drought stress and to nematode (Meloidogyne arenaria) challenge we conducted comprehensive transcriptome analyses using sequencing data from Sanger, 454 and Illumina HI-SEQ technologies. In silico analysis revealed that several genes were significantly up- or down-regulated in the stressed or control conditions. Differentially expressed candidate genes related to abiotic and biotic stresses were further selected for validation through RT-qPCR. Among these, expansin, aquaporin, dehydrin, chaperone, nitrilase, transcription factors, resistance protein MG13, resveratrol synthase genes revealed high levels of differential expression in stressed plants. This data confirms the relationship of these genes with drought stress response or root-knot nematode infection in wild Arachis species. The identification of candidate genes for resistance to abiotic and biotic stresses can provide additional resources for peanut breeding and transgenic approaches.
Thesagro:	Amendoim Arachis Hypogaea
NAL Thesaurus:	Peanuts genes
Palabras clave:	Diversidade genética Cultivo
Tipo de Material:	Resumo em anais e proceedings
Acceso:	openAccess
Aparece en las colecciones:	Resumo em anais de congresso (CPATSA)