Title:	Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil.
Authors:	SILVA, M. R. ROCHA, A. S. COSTA, R. R. ALENCAR, A. P. OLIVEIRA, V. M. de FONSECA JÚNIOR, A. A. SALES, M. L. ISSA, M. O. SOARES FILHO, P. M. PEREIRA, O. T. V. SANTOS, E. C. MENDES, R. S. FERREIRA, A. M. de J. MOTA, P. M. P. C. SUFFYS, P. N. GUIMARAES, M. D. C.
Affiliation:	MARCIO ROBERTO SILVA, CNPGL; Adalgiza da Silva Rocha, FIOCRUZ RJ; Ronaldo Rodrigues da Costa, FHEMIG Juiz de Fora; Andrea Padilha de Alencar, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; VANIA MARIA DE OLIVEIRA, CNPGL; Antônio Augusto Fonseca Júnior, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Mariana Lázaro Sales, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Marina de Azevedo Issa, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Paulo Martins Soares Filho, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Omara Tereza Vianello Pereira, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Eduardo Calazans dos Santos, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Rejane Silva Mendes, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Ângela Maria de Jesus Ferreira, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Pedro Moacyr Pinto Coelho Mota, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Philip Noel Suffys, FIOCRUZ RJ; Mark Drew Crosland Guimarães, UFMG.
Date Issued:	2013
Citation:	Memórias do Instituto Oswaldo Cruz, v. 108, n. 3, p. 321-327, 2013.
Description:	In this cross-sectional study, mycobacteria specimens from 189 tuberculosis (TB) patients living in an urban area in Brazil were characterised from 2008-2010 using phenotypic and molecular speciation methods (pncA gene and oxyR pseudogene analysis). Of these samples, 174 isolates simultaneously grew on Löwenstein-Jensen (LJ) and Stonebrink (SB)-containing media and presented phenotypic and molecular profiles of Mycobacterium tuberculosis, whereas 12 had molecular profiles of M. tuberculosis based on the DNA analysis of formalin-fixed paraffin wax-embedded tissue samples (paraffin blocks). One patient produced two sputum isolates, the first of which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, and the second of which only grew on SB media and presented phenotypic profiles of Mycobacterium bovis. One patient provided a bronchial lavage isolate, which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, but had molecular profiles of M. bovis from paraffin block DNA analysis, and one sample had molecular profiles of M. tuberculosis and M. bovis identified from two distinct paraffin blocks. Moreover, we found a low prevalence (1.6%) of M. bovis among these isolates, which suggests that local health service procedures likely underestimate its real frequency and that it deserves more attention from public health officials.
Thesagro:	Mycobacterium Bovis
NAL Thesaurus:	Mycobacterium tuberculosis
Keywords:	Zoonotic tuberculosis
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPGL)