Título:	Reproductive parameters and the use of MOET in transgenic founder goat carrying the human granulocyte colony-stimulating factor (hG-CSF) gene.
Autoria:	MOURA, R. R. SOUZA-FABJAN, J. M. G. FONSECA, J. F. da MELO, C. H. S. SANCHEZ, D. J. D. VIEIRA, M. P. ALMEIDA, T. M. SEROVA, I. A. SEROV, O. L. PEREIRA, A. F. TEIXEIRA, D. I. A. MELO, L. M. FREITAS, V. J. F.
Afiliação:	Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; JEFERSON FERREIRA DA FONSECA, CNPC; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; Institute of Cytology and Genetics, Novosibirsk, Russia.; Institute of Cytology and Genetics, Novosibirsk, Russia.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.
Ano de publicação:	2014
Referência:	Animal Reproduction, v. 11, n. 1, p. 37-43, Jan./Mar. 2014.
Conteúdo:	Abstract: This study aimed to monitor estrous cycle parameters of a human granulocyte colony-stimulating factor (hG-CSF)-transgenic founder female goat and to perform superovulation and embryo recovery (surgical or transcervical method) for further transfer to recipients to quickly obtain offspring. Two experiments were performed using a transgenic (TF) and a non-transgenic (NTF) female. In experiment 1, three estrous cycles were monitored for the following parameters: estrus behavior, progesterone concentration and ovarian activity. In experiment 2, two superovulation/embryo recovery sessions were performed and the recovered embryos were transferred to previously prepared recipients. Data were compared by either t test or Fisher's exact test. The mean interval between natural estrus was 20.7 ± 0.6 and 19.7 ± 0.6 (P > 0.05) days for the TF and NTF, respectively. Progesterone concentrations and ovarian activity were normal and similar between goats. The ovulation rate was similar between TF and NTF (12.0 ± 1.4 vs. 18.0 ± 4.2 CL; P > 0.05). No significant differences in embryo recovery rate (P > 0.05) were observed between the surgical and transcervical methods for TF (69.2 vs. 72.7%) or NTF (100.0 vs. 86.7%). Sixteen embryos from the TF were transferred to recipients, and eight kids were born. Among these kids, the transgene was identified in three (two males and one female), resulting in a transgenesis rate of 37.5%. In summary, the TF is a true founder, since she proved fertility and capacity of transmitting the hG-CSF transgene to progeny, suggesting that the analyzed reproductive traits were not compromised by the presence of the transgene.
Thesagro:	Caprino Cabra Transferência de embrião Hormônio animal Progesterona Superovulação
NAL Thesaurus:	Embryo transfer Progesterone Superovulation Transgenesis
Palavras-chave:	Transgenia Goat
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPC)