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dc.contributor.authorMUNIZ, C. R.pt_BR
dc.contributor.authorSILVA, G. F. dapt_BR
dc.contributor.authorSOUZA JUNIOR, M. T.pt_BR
dc.contributor.authorFREIRE, F. das C. O.pt_BR
dc.contributor.authorKEMA, G. H. J.pt_BR
dc.contributor.authorGUEDES, M. I. F.pt_BR
dc.date.accessioned2014-05-13T01:35:27Z-
dc.date.available2014-05-13T01:35:27Z-
dc.date.created2014-05-12pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationGenetics and Molecular Research, v. 13, n. 2, p. 2906-2913, 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/985915pt_BR
dc.descriptionLasiodiplodia theobromae is a major pathogen of many different crop cultures, including cashew nut plants. This paper describes an efficient Agrobacterium tumefaciens-mediated transformation (ATMT) system for the successful delivery of T-DNA, transferring the genes of green fluorescent protein (gfp) and hygromycin B phosphotransferase (hph) to L. theobromae. When the fungal pycnidiospores were co-cultured with A. tumefaciens harboring the binary vector with hph-gfp gene, hygromycin-resistant fungus only developed with acetosyringone supplementation.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectCashew plantspt_BR
dc.subjectMediated transformationpt_BR
dc.titleAgrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2014-05-13T01:35:27Zpt_BR
dc.subject.thesagroAgrobacterium Tumefacienspt_BR
dc.subject.thesagroLasiodiplodia Theobromaept_BR
dc.subject.nalthesaurusgummosispt_BR
riaa.ainfo.id985915pt_BR
riaa.ainfo.lastupdate2014-05-12pt_BR
dc.identifier.doiDOI http://dx.doi.org/10.4238/2014.February.21.8pt_BR
dc.contributor.institutionCELLI RODRIGUES MUNIZ, CNPAT; GILVAN FERREIRA DA SILVA, CPAA; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE; FRANCISCO DAS CHAGAS O FREIRE, CNPAT; G. H. J. Kema, Biointeractions and Plant Health; M. I. F. Guedes, Universidade Estadual do Ceará.pt_BR
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