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Título: Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants.
Autor: MUNIZ, C. R.
SILVA, G. F. da
SOUZA JUNIOR, M. T.
FREIRE, F. das C. O.
KEMA, G. H. J.
GUEDES, M. I. F.
Afiliación: CELLI RODRIGUES MUNIZ, CNPAT; GILVAN FERREIRA DA SILVA, CPAA; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE; FRANCISCO DAS CHAGAS O FREIRE, CNPAT; G. H. J. Kema, Biointeractions and Plant Health; M. I. F. Guedes, Universidade Estadual do Ceará.
Año: 2014
Referencia: Genetics and Molecular Research, v. 13, n. 2, p. 2906-2913, 2014.
Descripción: Lasiodiplodia theobromae is a major pathogen of many different crop cultures, including cashew nut plants. This paper describes an efficient Agrobacterium tumefaciens-mediated transformation (ATMT) system for the successful delivery of T-DNA, transferring the genes of green fluorescent protein (gfp) and hygromycin B phosphotransferase (hph) to L. theobromae. When the fungal pycnidiospores were co-cultured with A. tumefaciens harboring the binary vector with hph-gfp gene, hygromycin-resistant fungus only developed with acetosyringone supplementation.
Thesagro: Agrobacterium Tumefaciens
Lasiodiplodia Theobromae
NAL Thesaurus: gummosis
Palabras clave: Cashew plants
Mediated transformation
DOI: DOI http://dx.doi.org/10.4238/2014.February.21.8
Tipo de Material: Artigo de periódico
Acceso: openAccess
Aparece en las colecciones:Artigo em periódico indexado (CPAA)

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