Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/993623
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dc.contributor.authorPERINI, P.pt_BR
dc.contributor.authorPASQUALI, G.pt_BR
dc.contributor.authorMARGIS-PINHEIRO, M.pt_BR
dc.contributor.authorOLIVEIRA, P. R. D. dept_BR
dc.contributor.authorREVERS, L. F.pt_BR
dc.date.accessioned2016-02-22T12:38:26Z-
dc.date.available2016-02-22T12:38:26Z-
dc.date.created2014-08-28pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationMolecular Breeding, mar. 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/993623pt_BR
dc.descriptionApple (Malus 9 domestica Borkh.) is the most important deciduous tree fruit crop grown around the world. Comparisons of gene expression profiles from different tissues, conditions or cultivars are valuable scientific tools to better understand the gene expression changes behind important silvicultural and nutritional traits. However, the accuracy of techniques employed to access gene expression is dependent on the evaluation of stable reference genes for data normalization to avoid statistical significance undue or incorrect conclusions. The objective of this work was to select the best genes to be used as references for gene expression studies in apple trees by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Vegetative and reproductive tissues of the apple ??Gala?? cultivar were evaluated during their seasonal cycle of growth and dormancy. The expression of 23 traditional housekeeping genes or genes suggested as constitutive by microarray data was investigated. Tested combinations of primers allowed the specific amplification and the generation of suitable efficiency curves for gene expression studies by RT-qPCR. Gene stability was determined by two different statistical descriptors, geNorm and Norm-Finder. The known variable PAL gene expression was used to validate selected normalizers. Results obtained allowed us to conclude that MDH, SAND, THFS, TMp1 and WD40 are the best reference genes to accurately normalize the relative transcript abundances using RT-qPCR in various tissues of apple.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectGalapt_BR
dc.subjectExpressão genéticapt_BR
dc.subjectGenes de referenciapt_BR
dc.subjectRT-qPCRpt_BR
dc.titleReference genes for transcriptional analysis of flowering and fruit ripening stages in apple (Malus 3 domestica Borkh.).pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2019-04-02T11:11:11Zpt_BR
dc.subject.thesagroMaçãpt_BR
dc.subject.thesagroGenetica vegetalpt_BR
dc.description.notesDOI 10.1007/s11032-014-0078-3pt_BR
riaa.ainfo.id993623pt_BR
riaa.ainfo.lastupdate2019-04-02 -03:00:00pt_BR
dc.contributor.institutionPâmela Perini; Giancarlo Pasquali; Márcia Margis-Pinheiro; PAULO RICARDO DIAS DE OLIVEIRA, CNPUV; LUIS FERNANDO REVERS, CNPUV.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPUV)

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