Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/996228
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dc.contributor.authorSANTOS, R. dos S.por
dc.contributor.authorMURUCI, L. N. M.por
dc.contributor.authorSANTOS, L. O.por
dc.contributor.authorANTONIASSI, R.por
dc.contributor.authorSILVA, J. P. L. dapor
dc.contributor.authorDAMASO, M. C. T.por
dc.contributor.otherRegiane Ribeiro dos Santos, Universidade Federal Rural do Rio de Janeiro; Livia Nolasco Macedo Muruci, Universidade Federal Rural do Rio de Janeiro; Lucielen Oliveira Santos, Universidade Federal Rural do Rio de Janeiro; ROSEMAR ANTONIASSI, CTAA; JANINE PASSOS LIMA DA SILVA, CTAA; MONICA CARAMEZ TRICHES DAMASO, CNPAE.por
dc.date.accessioned2014-10-01T11:11:11Zpor
dc.date.available2014-10-01T11:11:11Zpor
dc.date.created2014-10-01por
dc.date.issued2014por
dc.identifier.other12308por
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/996228por
dc.descriptionLipases are enzymes that catalyze the hydrolysis of the ester linkage in a triacylglycerol oil-water interface, while in non-aqueous environments they catalyze reverse reactions (esterification, transesterification and interesterification). They can be produced by a solid state fermentation (SSF) process adding value to agro-industrial residues as alkaline soapstocks that can be used as a lipid substrate for lipase production. The objective of this study was to characterize the soapstocks of canola, sunflower and corn and use these byproducts for fungal lipase production using the mutant strain 11T53A14 of Aspergillus niger. The work was carried out following 24-1 and 23 experimental designs in the presence and absence of inducers, respectively, aiming to evaluate the influence of some variables in the lipase production. The production by SSF was carried out in aerated columns incubated at 32°C. Among the soapstocks evaluated, the sunflower soapstock was the best inducer for enzyme production, with 201 U/gdm (gram of dry medium) using 108 spores/gm (gram of medium) inoculated on wheat bran humidified with 80 mL of liquid (ammonium sulfate solution) that corresponds to a final concentration of 0.5% nitrogen, and 3% inducer. The best lipase activity obtained without soapstock was 182 U/gdm under the same processing conditions as the experiment using soapstock. Although the results with the highest lipase activity with sunflower soapstock and without inducer are close, the Tukey test showed a significant difference of these results, which proves the importance of lipid substrate to improve the lipase activity.por
dc.description.uribitstream/item/109353/1/2014-034.pdfpor
dc.languageenpor
dc.language.isoengeng
dc.publisherJournal of Food and Nutrition Research, v. 2, n. 9, p. 561-566, 2014.por
dc.relation.ispartofEmbrapa Agroindústria de Alimentos - Artigo em periódico indexado (ALICE)por
dc.rightsopenAccesspor
dc.subjectFungos filamentosospor
dc.subjectFermentação em estado sólidopor
dc.subjectReator de colunapor
dc.subjectResíduos agroindustriais.por
dc.titleCharacterization of different oil soapstocks and their application in the lipase production by Aspergillus niger under solid state fermentation.por
dc.typeArtigo em periódico indexado (ALICE)por
dc.date.updated2016-02-15T11:11:11Zpor
dc.subject.thesagroLípase.por
dc.ainfo.id996228por
dc.ainfo.lastupdate2016-02-15por
dc.identifier.doi10.12691/jfnr-2-9-6por
Appears in Collections:Artigo em periódico indexado (CTAA)

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