Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/997589
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dc.contributor.authorSANTOS, R. R. dospt_BR
dc.contributor.authorMURUCI, L. N. M.pt_BR
dc.contributor.authorDAMASO, M. C. T.pt_BR
dc.contributor.authorSILVA, J. P. L. dapt_BR
dc.contributor.authorSANTOS, L. O.pt_BR
dc.date.accessioned2014-10-17T11:11:11Zpt_BR
dc.date.available2014-10-17T11:11:11Zpt_BR
dc.date.created2014-10-17pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationJournal of Food and Nutrition Research, v. 2, n. 10, p. 659-663, 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/997589pt_BR
dc.descriptionLipases have various industrial applications and their production by filamentous fungi in solid state fermentation (SSF) process is of interest. In this work the lipase enzyme production by the mutant Aspergillus niger 11T51A14, which is a well-known lipase producer was studied using wheat bran as substrate. Microorganism used in fermentation process was inoculated at a concentration of 107 spores/gm into the medium containing nitrogen (ammonium sulfate), sunflower soapstock and wheat bran. The SSF process for enzyme production was carried out in aerated columns immersed in a water bath at 32ºC for 72 h. The process was studied using a central composite rotatable design 22, where variables tested were the nitrogen concentration (0.32-0.88 % w/w) and the volume of liquid (60.9-89.1 mL). The lipase activity was analyzed in enzymatic extracts. The wheat bran, in the fermentation medium, acted as a carbohydrate source and as an inducer for the lipase production. The maximum enzyme activity found was 153.4 U/gdm, with a nitrogen concentration of 0.6 % and 89.1 mL of volume of liquid. The response surface model demonstrated that higher volume of liquid had a positive effect in lipase activity, while nitrogen concentration showed an optimal range (0.4-0.6%).pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectBorrapt_BR
dc.subjectFementação em estado sólidopt_BR
dc.subjectResíduo agroindustrialpt_BR
dc.titleLipase production by Aspergillus niger 11T53A14 in wheat bran using experimental design methodology.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-02-15T11:11:11Zpt_BR
dc.subject.thesagroFungopt_BR
riaa.ainfo.id997589pt_BR
riaa.ainfo.lastupdate2016-02-15pt_BR
dc.identifier.doijfnr-2-10-1pt_BR
dc.contributor.institutionRegiane Ribeiro dos Santos, UFRRJ; Lívia Nolasco Macedo Muruci, UFRRJ; MONICA CARAMEZ TRICHES DAMASO, CNPAE; JANINE PASSOS LIMA DA SILVA, CTAA; Lucielen Oliveira Santos, FURG.pt_BR
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