Título:	Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.
Autoria:	FAJARDO, T. V. M. NICKEL, O.
Afiliação:	THOR VINICIUS MARTINS FAJARDO, CNPUV; OSMAR NICKEL, CNPUV.
Ano de publicação:	2014
Referência:	Ciência Rural, Santa Maria, v. 44, n. 10, p. 1711-1714, out. 2014.
Conteúdo:	The viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was suffi ciently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specifi city was confi rmed by reaction with homologous viral cDNA, individually cloned for each virus.
Thesagro:	Maçã Doença de planta Hibridação
NAL Thesaurus:	Apple chlorotic leaf spot virus Apple mosaic virus Apple stem grooving virus Apple stem pitting virus
Palavras-chave:	ASGV ACLSV ASPV ApMV Macieira Polissonda
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPUV)