Título:	Knock-down of heat-shock protein 90 and isocitrate lyase gene expression reduced root-knot nematode reproduction.
Autor:	LOURENCO, I. T. SOUZA JÚNIOR, J. D. A. MARTINS-DE-SA, D. VIANA, A. A. B. CARNEIRO, R. M. D. G. TOGAWA, R. C. ALMEIDA-ENGLER, J. de BATISTA, J. A. N. SILVA, M. C. M. da FRAGOSO, R. da R. SA, M. F. G. de
Afiliación:	ISABELA TRISTAN LOURENCO, CENARGEN JOSÉ DIJAIR ANTONINO SOUZA JUNIOR, UNIVERSIDADE DE BRASÍLIA DIOGO MARTINS-DE-SA, UNIVERSIDADE DE BRASÍLIA ANTÔNIO AMÉRICO BARBOSA VIANA REGINA MARIA DECHECHI G CARNEIRO, CENARGEN ROBERTO COITI TOGAWA, CENARGEN JANICE DE ALMEIDA-ENGLER, INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE JOÃO AGUIAR NOGUEIRA BATISTA, UNIVERSIDADE DE MINAS GERAIS MARIA CRISTINA MATTAR DA SILVA, CENARGEN RODRIGO DA ROCHA FRAGOSO, CPAC MARIA FATIMA GROSSI DE SA, CENARGEN.
Año:	2015
Referencia:	Phytopathology, v. 105, n. 5, p. 628-637, 2015.
Descripción:	Crop losses caused by nematode infections are estimated to be valued at USD 157 billion per year. Meloidogyne incognita, a root-knot nematode (RKN), is considered to be one of the most important plant pathogens due to its worldwide distribution and the austere damage it can cause to a large variety of agronomically important crops. RNA interference (RNAi), a gene silencing process, has proven to be a valuable biotechnology alternative method for RKN control. In this study, the RNAi approach was applied, using fragments of M. incognita genes that encode for two essential molecules, heat-shock protein 90 (HSP90) and isocitrate lyase (ICL). Plant-mediated RNAi of these genes led to a significant level of resistance against M. incognita in the transgenic Nicotiana tabacum plants. Bioassays of plants expressing HSP90 dsRNA demonstrated a delay in gall formation and up to 46% reduction in eggs compared with wild-type plants. A reduction in the level of HSP90 transcripts was observed in recovered eggs from plants expressing dsRNA, indicating that gene silencing persisted and was passed along to first progeny. The ICL knock-down had no clear effect on gall formation but resulted in up to 77% reduction in egg oviposition compared with wild-type plants. Our data suggest that both genes may be involved in RKN development and reproduction. Thus, in this paper, we describe essential candidate genes that could be applied to generate genetically modified crops, using the RNAi strategy to control RKN parasitism.
Thesagro:	Nematoide Praga de planta Reprodução
Palabras clave:	Controle de pragas
Tipo de Material:	Artigo de periódico
Acceso:	openAccess
Aparece en las colecciones:	Artigo em periódico indexado (CENARGEN)