Title:	Comparison of the effects of anticoagulants used in blood collection to determine blood parameters of free-living stingrays from the Potamotrygon genus (Elasmobranchii: Potamotrygonidae).
Authors:	OLIVEIRA, A. T. de SANTOS, M. Q. de C. LEMOS, J. R. G. TAVARES-DIAS, M. MARCON, J. L.
Affiliation:	ADRIANO TEIXEIRA DE OLIVEIRA, IFAM, Campus Presidente Figueiredo, Manaus, AM.; MÁRCIO QUARA DE CARVALHO SANTOS, IFAM, Campus Coari, Manaus, AM.; JEFFERSON RAPHAEL GONZAGA LEMOS, FAMETRO, Manaus, AM.; MARCOS TAVARES-DIAS, CPAF-AP; JAYDIONE LUIZ MARCON, UFAM, Manaus, AM.
Date Issued:	2015
Citation:	Biota Amazônia, Macapá, v. 5, n. 3, p. 55-58, 2015.
Description:	This study assessed the effectiveness of three anticoagulants in blood parameters of "cururu" stingrays Potamotrygon cf . histrix. Blood from ten individuals were collected and diluted with anticoagulants EDTA 5% and 10%, heparin 2500 and 5000 UI and sodium citrate 3.2%. A blood sample without anticoagulant was also evaluated. The blood of samples without anticoagulant and with sodium citrate 3.2% coagulated in 20% and 30% of the cases, respectively. Clumps of cells were observed during erythrocyte counting in 30% of samples with EDTA 10%, 40% of samples with heparin 2500 IU and 60% of samples with heparin 5000 IU. No alterations were observed on the erythrogram of "cururu" stingrays with different anticoagulants, the values of plasma glucose were similar in all groups and total protein levels were lower in the samples with EDTA 5% and 10%. The use of sodium citrate 3.2% is not recommended for blood sample conservation of Potamotrygon cf . histrix. stingrays, but anticoagulants did not affect the parameters analyzed in the determination of plasma glucose and erythrogram. Therefore , these results indicate that in order to blood coagulation the samples should be collected directly with any of these anticoagulants concentrations.
Thesagro:	Hematologia Sangue
NAL Thesaurus:	hematology blood
Keywords:	Anticoagulante
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPAF-AP)