Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1062269
Unidade da Embrapa/Coleção:: Embrapa Gado de Corte - Artigo em periódico indexado (ALICE)
Data do documento: 31-Jan-2017
Tipo do Material: Artigo em periódico indexado (ALICE)
Autoria: SANCHES, C. C.
ROSINHA, G. M. S.
GALVÃO, C. E.
FEIJO, G. L. D.
ARAUJO, F. R.
SOARES, C. O.
Informaçães Adicionais: CRISTIANE C. SANCHES, UFMS; GRACIA MARIA SOARES ROSINHA, CNPGC; CLEER E. GALVÃO, UFMS; GELSON LUIS DIAS FEIJO, CNPGC; FLABIO RIBEIRO ARAUJO, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC.
Título: Polymorphisms in the prion protein gene of cattle breeds from Brazil.
Edição: 2016
Fonte/Imprenta: Pesquisa Veterinária Brasileira, Brasília, DF, v. 36, n. 11, p. 1059-1066, nov. 2016.
Idioma: en
Palavras-chave: PRNP
Indel
Conteúdo: One of the alterations that occur in the PRNP gene in bovines is the insertion/deletion (indel) of base sequences in specific regions, such as indels of 12-base pairs (bp) in intron 1 and of 23- bp in the promoter region. The deletion allele of 23 bp is associated with susceptibility to bovine spongiform encephalopathy (BSE) as well as the presence of the deletion allele of 12 bp. In the present study, the variability of nucleotides in the promoter region and intron 1 of the PRNP gene was genotyped for the Angus, Canchim, Nellore and Simmental bovine breeds to identify the genotype profiles of resistance and/or susceptibility to BSE in each animal. Genomic DNA was extracted for amplification of the target regions of the PRNP gene using polymerase chain reaction (PCR) and specific primers. The PCR products were submitted to electrophoresis in agarose gel 3% and sequencing for genotyping. With the exception of the Angus breed, most breeds exhibited a higher frequency of deletion alleles for 12 bp and 23 bp in comparison to their respective insertion alleles for both regions. These results represent an important contribution to understanding the formation process of the Brazilian herd in relation to bovine PRNP gene polymorphisms.
Thesagro: Doença da vaca louca
Polimorfismo
Bovino
NAL Thesaurus: Brazil
Bovine spongiform encephalopathy
Prions
Cattle
Ano de Publicação: 2016
Aparece nas coleções:Artigo em periódico indexado (CNPGC)

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