Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1065188
Research center of Embrapa/Collection: Embrapa Café - Artigo em periódico indexado (ALICE)
Date Issued: 2016
Type of Material: Artigo em periódico indexado (ALICE)
Authors: FREITAS, R. T. da
PAIVA, R.
SALES, T. S.
SILVA, D. P. C. da
REIS, M. V. dos
SOUZA, A. C. de
ROSA, S. D. V. F. da
Additional Information: RODRIGO THEREZAN DE FREITAS, UFLA; RENATO PAIVA, UFLA; THAIS SILVA SALES, UFLA; DIOGO PEDROSA CORRÊA DA SILVA, UFLA; MICHELE VALQUÍRIA DOS REIS, UFLA; ANA CRISTINA DE SOUZA, UFLA; STTELA DELLYZETE VEIGA F DA ROSA, SAPC.
Title: Cryopreservation of Coffea arabica L. zygotic embryos by vitrification.
Publisher: Notulac Botanicae Horti Agrobotanici Cluj-Napoca, v. 44, n. 2, p. 445-451, Dec. 2016.
Language: en
Keywords: Liquid nitrogen
PVS2
TTC test
Description: As a consequence of the difficulty in conventional coffee seed storage, biotechnological alternatives such as cryopreservation have been investigated. The objective of this study was to develop a protocol for the cryopreservation of Coffea arabica L. (cv. ?Catuaí Vermelho? - IAC 144) zygotic embryos by vitrification. For the cryopreservation study, the embryos were immersed in Plant Vitrification Solution 2 at different times (0, 10, 25, 50, 100, and 250 min) and two temperatures (0 and 25 °C). Subsequently, the best thawing time was determined in a water bath (1, 3, 5 minutes or directly in Recovery Solution). An anatomical study was conducted on non-stored and stored embryos, with or without the use of Plant Vitrification Solution 2. The immersion in cryoprotectant solution for 100 min at 0 °C allows embryo cryopreservation. Embryos can be directly thawed in Recovery Solution after storage in liquid nitrogen. It was observed that Plant Vitrification Solution 2 reduced internal water content in the cells, allowing subsequent embryo growth resumption.
NAL Thesaurus: Ex situ conservation
Histology
Thawing
Data Created: 2017-02-22
Appears in Collections:Artigo em periódico indexado (SAPC)

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