Title:	Copaiba oil-resin (Copaifera reticulata Ducke) modulates the inflammation in a model of injury to rats' tongues.
Authors:	TEIXEIRA, F. B. SILVA, R. de B. LAMEIRA, O. A. WEBBER, L. P. COUTO, R. S. D. MARTINS, M. D. LIMA, R. R.
Affiliation:	Francisco Bruno Teixeira, UFPA; Raíra de Brito Silva, UFPA; OSMAR ALVES LAMEIRA, CPATU; Liana Preto Webber, UFRGS; Roberta Souza D'Almeida Couto, UFPA; Manoela Domingues Martins, UFRGS; Rafael Rodrigues Lima, UFPA.
Date Issued:	2017
Citation:	BMC Complementary and Alternative Medicine, v. 17, n. 1, article 313, June 2017.
Description:	A method for the separation, identification and quantification of 24 phenolic compounds using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was developed and validated. Six species of traditional medicinal plants from the Brazilian Amazon region were studied (Mansoa alliacea, Bauhinia variegata var. variegata, Bauhinia variegata var. alboflava, Connarus perrottetii var. angustifolius, Cecropia obtusa and Cecropia palmata). The analytes were separated by a reversed-phase SB-C18 column (2.1 × 50 mm, 1.8 μm) using a gradient elution of 7 min composed of 0.1% acetic acid in water (v/v) and acetonitrile, at a constant flow rate of 0.8 mL min−1. The limit of detection for the analytes ranged between 0.5 and 130.3 μg L−1. Intra- and inter-day precision showed satisfactory results and the recoveries obtained for the 24 analytes varied between 91.7 and 111.9% for most of the evaluated matrices. Quantifications were performed with the standard addition method (r > 0.99) using the data acquired in a multiple reaction monitoring (MRM) mode. Rutin, apigenin, vanillic acid, caffeic acid, ferulic acid, p-coumaric acid and trans-cinnamic acid were found in all the studied plants. However, we highlight the high contents of rutin in varieties of Bauhinia variegata (up to 2.52 mg g−1 of plant), chlorogenic acid in species of genus Cecropia (up to 0.57 mg g−1 of plant) and catechin in Connarus perrottetii var. angustifolius (1.77 mg g−1 of plant).
Thesagro:	Copaíba Planta Medicinal
Keywords:	Copaifera Reticulata Ducke
DOI:	10.1186/s12906-017-1820-2
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPATU)