Title:	Comparison of volatile profile and antioxidant activity of Piper divaricatum G. Meyer (Piperaceae) using cuttings and cell tissue.
Authors:	CORPES, R. MONTEIRO, L. ANDRADE, E. H. MAIA, J. G. MENEZES, I. C. de SETZER, W. N. RAMOS, A. R. SILVA, J. K. R. da
Affiliation:	Rosana Corpes, UFPA; Lidiene Monteiro, UFPA; Eloísa H. Andrade, UFPA; José G. Maia, UFPA; ILMARINA CAMPOS DE MENEZES, CPATU; William N. Setzer, University of Alabama/Aromatic Plant Research Center; Alessandra R. Ramos, Universidade Federal do Sul e Sudeste do Pará; Joyce K. R. da Silva, UFPA.
Date Issued:	2019
Citation:	Journal of the Brazilian Chemical Society, v. 30, n. 11, p. 2291-2298, Nov. 2019.
Description:	In this study, P. divaricatum (Piperaceae) plants were subcultured by cuttings and cell tissue in order to carry out a comparison of the volatile profile, phenolic compounds, and antioxidant activity. Propagation by cuttings used vermiculite substrate and Murashige-Skoog (MS) medium in the absence of growth regulators and rooting started at 15 days of growth providing a higher number of plants after 90 days. In vitro propagation was performed using shoot apices as explants in MS media supplemented with 0.5 mg mL-1 BA (6-benzyladenine). Volatile profiles analyzed by gas chromatography-mass spectrometry (GC-MS) showed as main compounds methyl eugenol, E-?-ocimene, and ?-elemene in the in vivo and in vitro cultures. Phenolic contents determined by the Folin-Ciocalteu method had no significant difference at the end of 90 days of growth displaying a good linear correlation with antioxidant activity and phenylpropanoids amounts (r > 0.7). However, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging was higher for in vitro plants grown in comparison to in vivo plants cultured.
Thesagro:	Propagação Vegetativa Micropropagação Antioxidante Piperaceae
DOI:	https://doi.org/10.21577/0103-5053.20190128
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPATU)