Title:	Molecular characterizaion and core collection evaluation of Manihot esculenta Crantz.
Authors:	COSTA, N. A. AZÊVEDO, H. S. F. da S. SILVA, L. M. da CUNHA, E. F. M. SIVIERO, A. CAMPOS, T. de
Affiliation:	NATHALIA ALMEIDA COSTA, Universidade Federal do Acre (Ufac); HELLEN SANDRA FREIRES DA SILVA AZÊVEDO, Rede Bionorte; LUCIELIO MANOEL DA SILVA, CPAF-AC; ELISA FERREIRA MOURA CUNHA, CPATU; AMAURI SIVIERO, CPAF-AC; TATIANA DE CAMPOS, CPAF-AC.
Date Issued:	2020
Citation:	Bioscience Journal, v. 36, p. 22-35, Nov./Dec. 2020. Supp. 1.
Description:	Cassava is one of the most important subsistence crops in tropical regions. It is necessary to preserve and to know the genetic diversity existent for the adequate use of genetic resources. The evaluation of genetic diversity among genotypes results in information about potential parents in breeding programs, allows duplicates identification, and facilitates germplasm exchange between research institutions. The objective of this study was to characterize the genetic diversity of cassava accessions of North Brazil region. A total of 106 accessions were analyzed using ten microsatellite markers. The genetic parameters estimated were: expected heterozygosity (HE), observed heterozygosity (HO) and polymorphic information content (PIC). Clustering was performed using the UPGMA and Neighbor-Joining (NJ) method. Bayesian analysis, analysis of principal coordinates and identification of a core collection were also used. The ten loci amplified 8,40 alleles on average. The average heterozygosity estimates were: HE = 0.71, HO = 0.58 and PIC = 0.72. Genetic distances ranged from 0.158 to 0.908. Six (5,66%) accesses were redundant. Clustering and dispersion analysis didn?t differentiate bitter from sweet cassava, and there wasn?t correlation between groups and collect origin. The core collection consisted of 22 individuals that represented 94% of total allelic diversity and 20,75% of the base collection. The results indicate high dissimilarity between the accessions and allowed the detection of redundant genotypes, showing the use of genetic markers as informative tools for the management of collections.
Thesagro:	Mandioca Manihot Esculenta Melhoramento Genético Vegetal Variação Genética Marcador Genético
NAL Thesaurus:	Cassava Plant breeding Genetic variation Microsatellite repeats Heterozygosity
Keywords:	Manioc Yuca Marcador microssatélite Heterozigosidade Método UPGMA Método Neighbor-Joining Análise bayesiana Bayesin analysis Fitomejoramiento Variación genetica Repeticiones de microsatélite Heterocigosidad Acre Amazonas Roraima São Paulo
ISSN:	1981-3163
DOI:	http://dx.doi.org/ BJ-v36n0a2020-48247
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPAF-AC)