Title:	Cervical relaxation protocol in sheep decreases Oct-4 and Nanog gene expression temporarily.
Authors:	SANTOS, J. D. R. BATISTA, R. I. T. P. TAIRA, A. R. SOUZA-FABJAN, J. M. G. ESPÍRITO SANTO, C. G. do BRAIR, V. L. MONTEIRO NETTO, M. BALARO, M. F. A. FONSECA, J. F. da BRANDÃO, F. Z.
Affiliation:	JULIANA DANTAS RODRIGUES SANTOS, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; AUGUSTO RYONOSUKE TAIRA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JOANNA MARIA GONÇALVES SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; CAROLINE GOMES DO ESPÍRITO SANTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; VIVIANE LOPES BRAIR; MARINA MONTEIRO NETTO; MÁRIO FELIPE ALVAREZ BALARO; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.
Date Issued:	2020
Citation:	Animal Reproduction, v. 17, n. 3, 2020. Edição dos Abstracts of the 34nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE), 2020 (online).
Description:	Hormonal protocols aiming at dilation of the sheep cervix were developed to allow access to the uterus. Despite the proven efficacy of these protocols, little is known about these drugs used on embryonic quality. Thus, this study evaluated the influence of the hormonal protocol of cervical dilation (based on estradiol benzoate, d?cloprostenol and oxytocin) on the expression profile of genes related to incidence of apoptosis (Bax and Bcl-2), stress (Hsp90 and PRDX1) and pluripotency maintenance (Oct-4 and NANOG). For this, two experiments were conducted. In conclusion, the cervical relaxation protocol based on the administration of estradiol benzoate, d-cloprostenol and oxytocin may temporarily alter the expression of NANOG and OCT4 genes in surgically recovered sheep embryos.
NAL Thesaurus:	Sheep Animal reproduction Benzoates Cloprostenol Oxytocin
Keywords:	Effects
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (CNPC)