Título:	First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product.
Autor:	ROZENTAL, T. FARIA, L. S. de FORNEAS, D. GUTERRES, A. L. RIBEIRO, J. B. ARAUJO, F. R. LEMOS, E. R. S. SILVA, M. R.
Afiliación:	TATIANA ROZENTAL, Fundação Oswaldo Cruz LETÍCIA SCAFUTTO DE FARIA, Universidade Federal de Juiz de Fora DANIELLE FORNEAS, Fundação Oswaldo Cruz ALEXANDRO GUTERRES, Fundação Oswaldo Cruz JOAO BATISTA RIBEIRO, CNPGL FLABIO RIBEIRO DE ARAUJO, CNPGC ELBA REGINA SAMPAIO LEMOS, Fundação Oswaldo Cruz MARCIO ROBERTO SILVA, CNPGL.
Año:	2020
Referencia:	The Brazilian Journal of Infectious Diseases, v. 24, n. 3, p. 208-212, 2020.
Descripción:	Global publications on Q fever have increased after the 2007 epidemic in the Netherlands. However, the epidemiology of Q fever/coxiellosis in Brazil is still poorly understood. Accordingly, there have been few studies investigating the presence of Coxiella burnetii in dairy products around the world, especially in Brazil, where consumption of fresh cheese made from raw-milk is very high. Objective: This study was a random survey to assess the prevalence of C. burnetii by PCR in traditional Minas artisanal cheese from the Serro microregion, Brazil, which is manufactured from bovine raw-milk. Methods: DNA extracted from 53 cheese samples were analyzed by nested PCR with C. burnetii-specific primers and the products confirmed by DNA sequencing. Results: Out of the 53 cheese samples five (9.43%) were C. burnetii DNA-positive, each coming from one of the respective randomly selected manufacturing agroindustries. Based on our results, it is estimated that 1.62 tons/day of ready-to-eat cheese made from raw-milk from a total of 16.2 tons produced daily in the study region are contaminated with C. burnetii.
Thesagro:	Leite Queijo de Minas Zoonose Segurança Alimentar
NAL Thesaurus:	Coxiella burnetii Food safety
Palabras clave:	Queijo mineiro artesanal
DOI:	https://doi.org/10.1016/j.bjid.2020.05.003
Tipo de Material:	Artigo de periódico
Acceso:	openAccess
Aparece en las colecciones:	Artigo em periódico indexado (CNPGL)